JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB72813

FITC Anti-Cytokeratin 18 抗体 [DC-10]

FITC Anti-Cytokeratin 18 antibody [DC-10]

Be the first to review this product! Submit a review

|

(3 Publications)

Anti-Cytokeratin 18 antibody [DC-10] conjugated to FITC - suitable for intracellular flow cytometry, ICC/IF, and reacts with human samples. Clone [DC-10] is a mouse monoclonal antibody cited in 5 publications.

別名を表示する

CYK18, PIG46, KRT18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, Keratin-18, CK-18, K18

3 Images
Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Overlay histogram showing MCF-7 cells stained with ab72813 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72813, 0.5μg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 (FITC) (2μg/1x106 cells) for 30 min at 22°C. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

ICC/IF image of ab72813 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72813, 1µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Cytokeratin 18 antibody [DC-10] (AB72813)

Flow cytometry analysis (intracellular staining) of HeLa cells with ab72813 at 5 μg/ml.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

DC-10

アイソタイプ

IgG1

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra)

applications

免疫原

Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information.

P05783

style
left-column

代替製品

Primary Antibodies

AB206091

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [EPR1626] (AB206091)

  • 0
  • 0
Primary Antibodies

AB133302

Anti-Cytokeratin 18 antibody [EPR1611]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 18 antibody [EPR1611] (AB133302)

  • 0
  • 0
Primary Antibodies

AB181597

Anti-Cytokeratin 18 antibody [EPR17347]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR17347] (AB181597)

  • 5
  • 3
Primary Antibodies

AB133263

Anti-Cytokeratin 18 antibody [EPR1626]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [EPR1626] (AB133263)

  • 3
  • 3
Primary Antibodies

AB32118

Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 antibody [E431-1] (AB32118)

  • 3
  • 6
Primary Antibodies

AB194124

Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [E431-1] (AB194124)

  • 0
  • 0

組合せ製品

Assay Kits

AB254515

Human Cytokeratin 18 Fragment ELISA Kit (CK18-30)

Recombinant|SimpleStep

ELISA - Human Cytokeratin 18 Fragment ELISA Kit (CK18-30) (AB254515)

  • 0
  • 0
Primary Antibodies

AB192467

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

  • 5
  • 5
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB192643

Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EP1580Y] (AB192643)

  • 4
  • 1
Primary Antibodies

AB237385

Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 647 Anti-EpCAM antibody [EPR20533-63] (AB237385)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.5-1 µg/mL", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "<p></p>" }, "Cow": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Dog": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Hamster": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Pig": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1-5 g/mL", "FlowCytIntra-species-notes": "<p></p>" }, "Sheep": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 g/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Size-exclusion chromatography
精製に関する特記事項
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.
Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Also acts downstream of ROCK kinase activation as part of a positive feedback mechanism in response to cellular mechanical stress loading (PubMed : 26823019). Organization and orientation of KRT18 filaments are responsible for the properly elongated morphology of epithelial tubules (By similarity). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
See full target information KRT18
style
left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 17:3799-3807 PubMed30881500

2019

Isolation of circulating tumor cells and detection of EGFR mutations in patients with non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Jingying Nong,Jinghui Wang,Zhuohong Yan,Ling Yi,Xin Gao,Zhidong Liu,Hongtao Zhang,Shucai Zhang

International journal of oncology : PubMed28350107

2017

A liquid biopsy-based method for the detection and quantification of circulating tumor cells in surgical osteosarcoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Haoqiang Zhang,Peng Gao,Xin Xiao,Michal Heger,Lei Geng,Bo Fan,Yulin Yuan,Chen Huang,Guojing Chen,Yao Liu,Yongchen Hu,Xiuchun Yu,Sujia Wu,Ling Wang,Zhen Wang

Journal of cell science 124:1644-54 PubMed21525033

2011

Phagocytosis of dying tumor cells by human peritoneal mesothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Britta Janina Wagner,Dennis Lindau,Dagmar Ripper,York-Dieter Stierhof,Jörg Glatzle,Maria Witte,Henning Beck,Hildegard Keppeler,Kirsten Lauber,Hans-Georg Rammensee,Alfred Königsrainer
View all publications
style
left-column
style
left-column
style
right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com