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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins
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FITC Anti-Cytokeratin 14 抗体 [LL002] (ab77684)

  • Datasheet
  • SDS
Reviews (6)Q&A (1)References (13)

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Flow Cytometry - FITC Anti-Cytokeratin 14 antibody [LL002] (ab77684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Cytokeratin 14 antibody [LL002] (ab77684)

Key features and details

  • FITC Mouse monoclonal [LL002] to Cytokeratin 14
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG3

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free Unconjugated

こちらの製品もご検討ください

標識
Product image
FITC Conjugation Kit - Lightning-Link® (ab102884)
一次抗体
Product image
Anti-RAD54 antibody [Rad54 4E3/1] (ab11055)
一次抗体
Product image
Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (ab206100)

関連製品

製品の概要

  • 製品名

    FITC Anti-Cytokeratin 14 antibody [LL002]
    Cytokeratin 14 一次抗体 製品一覧
  • 製品の詳細

    FITC Mouse monoclonal [LL002] to Cytokeratin 14
  • 由来種

    Mouse
  • 標識

    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide: GKVVSTHEQVLRTKN conjugated to Thyroglobulin, corresponding to C terminal amino acids 458-472 of Human Cytokeratin 14

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Store at +4°C.
  • バッファー

    Preservative: 0.065% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Ion Exchange Chromatography
  • 特記事項(精製)

    Ammonium sulphate precipitation followed by ion exchange chromatography.
  • ポリ/モノ

    モノクローナル
  • クローン名

    LL002
  • ミエローマ

    NS1
  • アイソタイプ

    IgG3
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

関連製品

  • Alternative Versions

    • Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)
    • Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab264498)
    • Alexa Fluor® 555 Anti-Cytokeratin 14 antibody [LL002] (ab320066)
    • Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [LL002] (ab320070)
    • Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [LL002] (ab321783)
    • Anti-Cytokeratin 14 antibody [LL002] (ab7800)
  • Isotype control

    • FITC Mouse IgG3 [PPV-07] - Isotype Control (ab91539)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab77684の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt
Use 10µl for 106 cells.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt
Use 10µl for 106 cells.

ターゲット情報

  • 機能

    The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
  • 組織特異性

    Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
  • 関連疾患

    Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.
    Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, although it is less severe.
    Defects in KRT14 are the cause of epidermolysis bullosa simplex autosomal recessive (AREBS) [MIM:601001]. AREBS is an intraepidermal epidermolysis bullosa characterized by localized blistering on the dorsal, lateral and plantar surfaces of the feet.
    Defects in KRT14 are the cause of Naegeli-Franceschetti-Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects.
    Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistent reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
  • 配列類似性

    Belongs to the intermediate filament family.
  • 細胞内局在

    Cytoplasm. Nucleus. Expressed in both as a filamentous pattern.
  • Target information above from: UniProt accession P02533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 3861 Human
    • Entrez Gene: 16664 Mouse
    • Entrez Gene: 287701 Rat
    • Omim: 148066 Human
    • SwissProt: P02533 Human
    • SwissProt: Q61781 Mouse
    • SwissProt: Q6IFV1 Rat
    • Unigene: 654380 Human
    • Unigene: 439898 Mouse
    • Unigene: 145931 Rat
    • Unigene: 153972 Rat
    see all
  • 別名

    • CK 14 antibody
    • CK-14 antibody
    • ck14 antibody
    • Cytokeratin 14 antibody
    • Cytokeratin-14 antibody
    • Cytokeratin14 antibody
    • Dowling Meara antibody
    • EBS3 antibody
    • EBS4 antibody
    • Epidermolysis bullosa simplex antibody
    • K14 antibody
    • K1C14_HUMAN antibody
    • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
    • Keratin 14 antibody
    • Keratin antibody
    • Keratin type I cytoskeletal 14 antibody
    • Keratin, type I cytoskeletal 14 antibody
    • Keratin-14 antibody
    • Keratin14 antibody
    • Koebner antibody
    • Krt 14 antibody
    • Krt14 antibody
    • NFJ antibody
    • OTTHUMP00000164624 antibody
    • type I cytoskeletal 14 antibody
    see all

画像

  • Flow Cytometry - FITC Anti-Cytokeratin 14 antibody [LL002] (ab77684)
    Flow Cytometry - FITC Anti-Cytokeratin 14 antibody [LL002] (ab77684)
    Overlay histogram showing A431 cells stained with ab77684 (red line). The cells were fixed with 4% paraformaldehyde (10 min)) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77684, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 FITC (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 80% methanol/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Cytokeratin 14 antibody [LL002] (ab77684)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - FITC Anti-Cytokeratin 14 antibody [LL002] (ab77684)
    IHC image of ab77684 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77684, neat, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (13)

ab77684 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab77684 は 13 報の論文で使用されています。

  • Royzman D  et al. Soluble CD83 improves and accelerates wound healing by the induction of pro-resolving macrophages. Front Immunol 13:1012647 (2022). PubMed: 36248909
  • Gray GK  et al. A human breast atlas integrating single-cell proteomics and transcriptomics. Dev Cell 57:1400-1420.e7 (2022). PubMed: 35617956
  • Wei F  et al. Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway. Aging (Albany NY) 12:12002-12018 (2020). PubMed: 32570219
  • Szymanski L  et al. A Simple Method for the Production of Human Skin Equivalent in 3D, Multi-Cell Culture. Int J Mol Sci 21:N/A (2020). PubMed: 32629914
  • Crowell PD  et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell Rep 28:1499-1510.e6 (2019). PubMed: 31390564
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for FITC Anti-Cytokeratin 14 antibody [LL002]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 07 2021

Immunocytochemistry/ Immunofluorescence abreview for FITC Anti-Cytokeratin 14 antibody [LL002]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Primary NHEK)
Permeabilization
No
Specification
Primary NHEK
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 06 2021

Immunohistochemistry (Frozen sections) abreview for FITC Anti-Cytokeratin 14 antibody [LL002]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Ovarian cancer)
Permeabilization
No
Specification
Ovarian cancer
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 28 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cytokeratin 14 antibody [LL002] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH = 6
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 07 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 14 antibody [LL002] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Keratinocytes (HaCaT))
Permeabilization
No
Specification
Keratinocytes (HaCaT)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 17 2020

Immunohistochemistry (Frozen sections) abreview for Anti-Cytokeratin 14 antibody [LL002] (FITC)

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin (melanoma))
Permeabilization
No
Specification
Skin (melanoma)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Christian Ostalecki

Verified customer

投稿 May 30 2018

Question

Would like to try ab77684 with pig samples.

Read More

Abcam community

Verified customer

Asked on Aug 23 2012

Answer

DISCOUNT CODE: ***
Expiration date: December 24, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab77684 with pig samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Aug 23 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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  • 感染症
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