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AB24935

FITC Anti-CTLA4 抗体 [1B8]

FITC Anti-CTLA4 antibody [1B8]

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(2 Publications)

Armenian hamster Monoclonal CTLA4 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CTLA4.

別名を表示する

CD152, CTLA4, Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4

1 Images
Flow Cytometry - FITC Anti-CTLA4 antibody [1B8] (AB24935)
  • Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CTLA4 antibody [1B8] (AB24935)

Flow cytometry analysis staining CTLA4 in mouse splenocytes using ab24935 at a dilution of 1 ug/106 cells. Hamster anti-mouse CD152-FITC was used as an isotype control.

Key facts

宿主種

Armenian hamster

クローン性

Monoclonal

クローン番号

1B8

アイソタイプ

IgG

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human CTLA4.

P16410

特異性

ab24935 recognises mouse CD152/CTLA-4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18473'>ab18473</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Store in the dark
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CTLA-4 also known as CD152 is an immune checkpoint receptor with a molecular weight of approximately 34 kDa. This protein is expressed on the surface of T-cells especially after activation and sometimes on regulatory T-cells (Tregs). CTLA-4 competes with CD28 for binding to the same ligands CD80 and CD86 on antigen-presenting cells. Unlike CD28 which stimulates T-cell activation CTLA-4 sends inhibitory signals to downregulate immune responses. By doing this CTLA-4 helps maintain immune system homeostasis and prevents autoimmune reactions.
Biological function summary

The CTLA-4 protein functions as a critical regulator of the immune system. It interacts with CD80/CD86 in a complex that effectively transmits inhibitory signals to T-cells. CTLA-4 controls the amplitude of the initial activation of T-cells ensuring that the body's immune responses are kept under control. In regulatory T-cells CTLA-4 engagement contributes to their immunosuppressive functions through which they maintain tolerance to self-antigens and prevent overactive immune reactions.

Pathways

CTLA-4 involves itself in the adaptive immune response pathways specifically the regulation of T-cell activity. CTLA-4's interaction with CD80/CD86 modulates pathways associated with TCR (T-cell receptor) signaling. When CTLA-4 binds its ligands it recruits phosphatases such as SHP-2 that dephosphorylate signaling proteins leading to the downregulation of T-cell interaction. This pathway interaction exemplifies how CTLA-4 acts in concert with proteins like CD28 to finely tune immune responses.

CTLA-4 plays a role in autoimmune diseases and cancer. The dysregulation of CTLA-4 function can lead to autoimmune disorders where the immune system attacks the body's own tissue. In cancer tumor cells may manipulate CTLA-4 pathways to evade immune surveillance. Targeted therapies like anti-CTLA-4 antibodies such as ipilimumab have been developed to block CTLA-4 aiming to enhance the immune system’s ability to attack cancer cells. These treatments highlight the role of CTLA-4 in balancing immune activity and the potential to alter this balance for therapeutic benefit.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Inhibitory receptor acting as a major negative regulator of T-cell responses (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 18641304, PubMed : 28484017). Acts as a decoy receptor : the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 28484017).
See full target information CTLA4
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 20:4303-4314 PubMed31545427

2019

MicroRNA‑30a controls the instability of inducible CD4+ Tregs through SOCS1.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Zhou,Yongju Li,Jia Lu,Xiaowu Hong,Lin Xu

International immunology 21:1065-77 PubMed19651643

2009

PD1 blockade reverses the suppression of melanoma antigen-specific CTL by CD4+ CD25(Hi) regulatory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenshi Wang,Roy Lau,Daohai Yu,Weiwei Zhu,Alan Korman,Jeffrey Weber
View all publications
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代替製品

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組合せ製品

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