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FITC Anti-CD97 抗体 [MEM-180] (ab23490)

Submit a review Q&A (1)References (2)
Flow Cytometry - FITC Anti-CD97 antibody [MEM-180] (ab23490)

    Key features and details

    • FITC Mouse monoclonal [MEM-180] to CD97
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Isotype: IgG1

    製品の概要

    • 製品名

      FITC Anti-CD97 antibody [MEM-180]
      CD97 一次抗体 製品一覧

    • 製品の詳細

      FITC Mouse monoclonal [MEM-180] to CD97

    • 由来種

      Mouse

    • 標識

      FITC. Ex: 493nm, Em: 528nm

    • 特異性

      ab23490 recognises the human CD97 cell surface antigen, a 74-89kD glycoprotein expressed by monocytes and activated lymphocytes.

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      PHA-activated PBL.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab23490の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 20µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
    特記事項
    Flow Cyt
    Use 20µl for 106 cells.

    ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

    ターゲット情報

    • 機能

      Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.

    • 組織特異性

      Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.

    • 配列類似性

      Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.
      Contains 5 EGF-like domains.
      Contains 1 GPS domain.

    • ドメイン

      The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.
      Binding to chondroitin sulfate is mediated by the fourth EGF domain.

    • 翻訳後修飾

      Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.

    • 細胞内局在

      Cell membrane and Secreted > extracellular space.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ADGRE5 antibody
        • Adhesion G protein-coupled receptor E5 antibody
        • CD 97 antibody
        • CD_antigen=CD97 antibody
        • CD97 antibody
        • CD97 antigen antibody
        • CD97 antigen subunit beta antibody
        • CD97 molecule antibody
        • CD97_HUMAN antibody
        • Leukocyte antigen CD97 antibody
        • Seven span transmembrane protein antibody
        • Seven transmembrane helix receptor antibody
        • Seven transmembrane heterodimeric receptor associated with inflammation antibody
        • TM7LN 1 antibody
        • TM7LN1 antibody
        see all

      画像

      • Flow Cytometry - FITC Anti-CD97 antibody [MEM-180] (ab23490)
        Flow Cytometry - FITC Anti-CD97 antibody [MEM-180] (ab23490)
        Flow Cytometry analysis of human peripheral blood cells labeling CD97 with Anti-CD97 antibody [MEM-180] (FITC) (ab23490).

      参考文献 (2)

      ab23490 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab23490 は 2 報の論文で使用されています。

      • Tian H  et al. Effects of targeted CD97 immune epitopes small interference RNA on cellular biological behaviors in MDA-MB231 malignant breast cancer cell line. Am J Transl Res 9:4640-4651 (2017). PubMed: 29118924
      • Ghazvini M  et al. Cancer stemness in Apc- vs. Apc/KRAS-driven intestinal tumorigenesis. PLoS One 8:e73872 (2013). PubMed: 24069241

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      The customer is looking for anti human CD97 for FACS (fluorescent conjugated) that binds STALK region. Kindly advise if ab23490/ab33229 are suitable for this purpose. If not, please advise an alternative. Thanks in advance.

      Read More
      Answer

      Thank you for contacting us. Yes, according to the attached reference (PMID 15386373), the clone MEM-180 binds to the stalk region of CD97. I hope this helps, please let me know if you need any additional information or assistance.

      Read More

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