FITC Anti-CD90 / Thy1 抗体 [MRC OX-7] (ab226)
Key features and details
- FITC Mouse monoclonal [MRC OX-7] to CD90 / Thy1
- Suitable for: Flow Cyt (Intra), ICC
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD90 / Thy1 antibody [MRC OX-7]
CD90 / Thy1 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [MRC OX-7] to CD90 / Thy1 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. -
アプリケーション
適用あり: Flow Cyt (Intra), ICCmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Rabbit, Guinea pig -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC: PC12 cells. Flow Cyt (Intra): PC12 cells.
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特記事項
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
MRC OX-7 -
ミエローマ
NS1 -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
- HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
- PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab226の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC |
1/100.
This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min). |
特記事項 |
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Flow Cyt (Intra)
1/500. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
1/100. This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min). |
ターゲット情報
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機能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
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別名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
画像
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ab226 staining CD90 / Thy1 in undifferentiated PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI(shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8)
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ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min at 22°C.
Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
This antibody gave a positive signal in PC12 (undifferentiated) cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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Flow cytometry overlay histogram showing PC12 (undifferentiated) cells stained with ab226 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction, followed by the antibody (ab226) (1x 106 in 100µl at 1µg/ml (1/500)) for 30 min on ice.
Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab226 は 29 報の論文で使用されています。
- Zhao Y et al. Mesenchymal Stem Cells Ameliorate Fibrosis by Enhancing Autophagy via Inhibiting Galectin-3/Akt/mTOR Pathway and by Alleviating the EMT via Inhibiting Galectin-3/Akt/GSK3β/Snail Pathway in NRK-52E Fibrosis. Int J Stem Cells 16:52-65 (2023). PubMed: 35483714
- Nair RS et al. A porcine cholecystic extracellular matrix conductive scaffold for cardiac tissue repair. J Biomed Mater Res B Appl Biomater 110:2039-2049 (2022). PubMed: 35305082
- Zhao J et al. Bone Regeneration and Angiogenesis by Co-transplantation of Angiotensin II-Pretreated Mesenchymal Stem Cells and Endothelial Cells in Early Steroid-Induced Osteonecrosis of the Femoral Head. Cell Transplant 31:9636897221086965 (2022). PubMed: 35313737
- Zhang S et al. A stem cell-derived ovarian regenerative patch restores ovarian function and rescues fertility in rats with primary ovarian insufficiency. Theranostics 11:8894-8908 (2021). PubMed: 34522217
- Ma YS et al. Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4. Cell Death Discov 7:224 (2021). PubMed: 34455417