FITC Anti-CD86 抗体 [BU63] (ab77276)
![Flow Cytometry - FITC Anti-CD86 antibody [BU63] (ab77276)](https://www.abcam.co.jp/ps/products/77/ab77276/Images/ab77276-455678-FITC-AntiCD86-antibody-Flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD86 antibody [BU63] (ab77276)")
Key features and details
- FITC Mouse monoclonal [BU63] to CD86
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD86 antibody [BU63]
CD86 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [BU63] to CD86 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD86. B-lymphoblastoid cell line ARH 77
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ポジティブ･コントロール
- Human blood cells.
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特記事項
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA
0.2% (w/v) high-grade protease free BSA -
Concentration information loading... -
精製度
Size exclusion -
ポリ/モノ
モノクローナル -
クローン名
BU63 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
- APC Anti-CD86 antibody [BU63], prediluted (ab134385)
- Anti-CD86 antibody [BU63] - BSA and Azide free (ab213044)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- PE Anti-CD86 antibody [BU63] (ab77226)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77276の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
Use 20 μl per 100 μl of whole blood or 106 cells |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. Use 20 μl per 100 μl of whole blood or 106 cells |
ターゲット情報
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機能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
組織特異性
Expressed by activated B-lymphocytes and monocytes. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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別名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
画像
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Flow Cytometry - FITC Anti-CD86 antibody [BU63] (ab77276)
Separation of human monocytes (red-filled) from CD86 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab77276 (20 μl reagent / 100 μl of peripheral whole blood).
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![Flow Cytometry - FITC Anti-CD86 antibody [BU63] (ab77276)](https://www.abcam.co.jp/ps/products/77/ab77276/Images/ab77276-455677-FITC-AntiCD86-antibody-Flow-cytometry.jpg)
Flow Cytometry - FITC Anti-CD86 antibody [BU63] (ab77276)Flow cytometry surface staining pattern of human peripheral whole blood stained using ab77276 (20 μl reagent / 100 μl of peripheral whole blood).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab77276 は 11 報の論文で使用されています。
- Chen S et al. YY1 complex in M2 macrophage promotes prostate cancer progression by upregulating IL-6. J Immunother Cancer 11:N/A (2023). PubMed: 37094986
- He Y et al. Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs. Nat Commun 14:7639 (2023). PubMed: 37993431
- Liu Z et al. Low-intensity pulsed ultrasound reduces lymphedema by regulating macrophage polarization and enhancing microcirculation. Front Bioeng Biotechnol 11:1173169 (2023). PubMed: 37214283
- Wang Q et al. Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF-kB Signaling Pathway. Evid Based Complement Alternat Med 2021:8868527 (2021). PubMed: 33505510
- Shrestha P et al. Pomalidomide restores immune recognition of primary effusion lymphoma through upregulation of ICAM-1 and B7-2. PLoS Pathog 17:e1009091 (2021). PubMed: 33411730