FITC Anti-CD62L 抗体 [MEL-14] (ab24876)
![Flow Cytometry - FITC Anti-CD62L antibody [MEL-14] (ab24876)](https://www.abcam.co.jp/ps/products/24/ab24876/Images/ab24876-276589-anti-cd62l-antibody-mel-14-fitc-flow-cytometry.png "Flow Cytometry - FITC Anti-CD62L antibody [MEL-14] (ab24876)")
Key features and details
- FITC Rat monoclonal [MEL-14] to CD62L
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD62L antibody [MEL-14]
CD62L 一次抗体 製品一覧 -
製品の詳細
FITC Rat monoclonal [MEL-14] to CD62L -
由来種
Rat -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD62L.
Database link: 20343 -
ポジティブ･コントロール
- BLAB/c splenocytes for Immunofluorescent staining.
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特記事項
This antibody blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin -
Concentration information loading... -
精製度
Affinity purified -
一次抗体 備考
This antibody blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling. -
ポリ/モノ
モノクローナル -
クローン名
MEL-14 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24876の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
組織特異性
Expressed in B cell lines and T lymphocytes. -
配列類似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 20343 Mouse
- SwissProt: P18337 Mouse
- Unigene: 1461 Mouse
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別名
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
画像
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Flow Cytometry - FITC Anti-CD62L antibody [MEL-14] (ab24876)
Flow cytometry C57Bl/6 splenocytes labeling CD62L with ab24876 at 0.25µg (solid line), or Rat IgG2a isotype control at 0.25µg (dashed line).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab24876 は 2 報の論文で使用されています。
- Morrison VL et al. TLR-mediated loss of CD62L focuses B cell traffic to the spleen during Salmonella typhimurium infection. J Immunol 185:2737-46 (2010). Flow Cyt ; Mouse . PubMed: 20660707
- Reichert RA et al. Dual immunofluorescence studies of cortisone-induced thymic involution: evidence for a major cortical component to cortisone-resistant thymocytes. J Immunol 136:3529-34 (1986). PubMed: 3084633