FITC Anti-CD59 抗体 [MEM-43], prediluted (ab18237)
![Flow Cytometry - FITC Anti-CD59 antibody [MEM-43], prediluted (ab18237)](https://www.abcam.co.jp/ps/products/18/ab18237/Images/ab18237-288026-anti-cd59-antibody-mem-43-prediluted-fitc-flow-cytometry.jpg "Flow Cytometry - FITC Anti-CD59 antibody [MEM-43], prediluted (ab18237)")
Key features and details
- FITC Mouse monoclonal [MEM-43] to CD59, prediluted
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD59 antibody [MEM-43], prediluted
CD59 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [MEM-43] to CD59, prediluted -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
The antibody reacts with a well defined epitope (W40, R-53) on CD59 molecule. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD59. Human thymocytes and T lymphocytes
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: PBS, BSA -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
MEM-43 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18237の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 20µl for 106 cells.
20 µl for a 100 µl sample is recommended.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
|
Flow Cyt
Use 20µl for 106 cells. 20 µl for a 100 µl sample is recommended.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
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機能
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
関連疾患
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
配列類似性
Contains 1 UPAR/Ly6 domain. -
翻訳後修飾
N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
細胞内局在
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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参照データベース
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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別名
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
画像
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Flow Cytometry - FITC Anti-CD59 antibody [MEM-43], prediluted (ab18237)
Flow cytometry analysis staining CD59 in HL-60 cells using ab18237 at a 1μg/ml dilution. Sp2 cells were used as the negative control.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab18237 は 9 報の論文で使用されています。
- Cupino TL et al. Stability and bioactivity of chitosan as a transfection agent in primary human cell cultures: A case for chitosan-only controls. Carbohydr Polym 180:376-384 (2018). PubMed: 29103517
- Makkonen KE et al. 6-o- and N-sulfated syndecan-1 promotes baculovirus binding and entry into Mammalian cells. J Virol 87:11148-59 (2013). PubMed: 23926339
- Wickham SE et al. Mapping the Intermedilysin-Human CD59 Receptor Interface Reveals a Deep Correspondence with the Binding Site on CD59 for Complement Binding Proteins C8{alpha} and C9. J Biol Chem 286:20952-62 (2011). Functional Studies ; Human . PubMed: 21507937
- LaChapelle S et al. Intermedilysin-receptor interactions during assembly of the pore complex: assembly intermediates increase host cell susceptibility to complement-mediated lysis. J Biol Chem 284:12719-26 (2009). FRET ; Human . PubMed: 19293153
- Cebecauer M et al. Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 243:706-10 (1998). PubMed: 9500981
- Cinek T & Horejsí V The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases. J Immunol 149:2262-70 (1992). PubMed: 1382093
- Stefanová I et al. GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science 254:1016-9 (1991). PubMed: 1719635
- Forsberg UH et al. Gene for human CD59 (likely Ly-6 homologue) is located on the short arm of chromosome 11. Immunogenetics 30:188-93 (1989). PubMed: 2476389
- Stefanová I et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859