FITC Anti-CD44 抗体 [KM201] (ab25064)
Key features and details
- FITC Rat monoclonal [KM201] to CD44
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD44 antibody [KM201]
CD44 一次抗体 製品一覧 -
製品の詳細
FITC Rat monoclonal [KM201] to CD44 -
由来種
Rat -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
Recognises all isoforms of CD44/pgp-1 (Mr 80-95 kDa) -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD44. (C57BL/6 x DBA/2)F1 mouse bone marrow-derived stromal clone BMS2
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エピトープ
Reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. -
特記事項
ab25064 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Affinity purified -
一次抗体 備考
ab25064 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells. -
ポリ/モノ
モノクローナル -
クローン名
KM201 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25064の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 2µg for 106 cells.
ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use 2µg for 106 cells. ab18404 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
組織特異性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
配列類似性
Contains 1 Link domain. -
ドメイン
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻訳後修飾
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12505 Mouse
- SwissProt: P15379 Mouse
- Unigene: 423621 Mouse
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別名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab25064 は 8 報の論文で使用されています。
- Shirzeyli MH et al. Comparison of the efficacy of bone morphogenetic protein-4 on in vitro differentiation of murine adipose and bone marrow mesenchymal stem cells into primordial germ cells. Res Pharm Sci 17:123-133 (2022). PubMed: 35280837
- Meng Q & Qiu B Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression. Front Physiol 11:441 (2020). PubMed: 32528301
- Xue Y et al. Role of the HIF-1a/SDF-1/CXCR4 signaling axis in accelerated fracture healing after craniocerebral injury. Mol Med Rep 22:2767-2774 (2020). PubMed: 32945380
- Li G et al. Hypermethylation of microRNA-149 activates SDF-1/CXCR4 to promote osteogenic differentiation of mesenchymal stem cells. J Cell Physiol 234:23485-23494 (2019). PubMed: 31206187
- Shirzeyli MH et al. Bones Morphogenic Protein-4 and retinoic acid combined treatment comparative analysis for in vitro differentiation potential of murine mesenchymal stem cells derived from bone marrow and adipose tissue into germ cells. Microsc Res Tech 80:1151-1160 (2017). PubMed: 28921810
- Hosseinzadeh Shirzeily M et al. Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells. Iran J Reprod Med 11:965-76 (2013). Flow Cyt ; Mouse . PubMed: 24639722
- Purcell BP et al. Synergistic effects of SDF-1a chemokine and hyaluronic acid release from degradable hydrogels on directing bone marrow derived cell homing to the myocardium. Biomaterials 33:7849-57 (2012). Blocking . PubMed: 22835643
- Fagoonee S et al. Lack of plasma protein hemopexin dampens mercury-induced autoimmune response in mice. J Immunol 181:1937-47 (2008). Flow Cyt ; Mouse . PubMed: 18641331