FITC Anti-CD4 抗体 [OX-35] (ab239230)
Key features and details
- FITC Mouse monoclonal [OX-35] to CD4
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
FITC Anti-CD4 antibody [OX-35]
CD4 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [OX-35] to CD4 -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Rat -
免疫原
Tissue, cells or virus corresponding to CD4. MLR generated rat Th cells.
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エピトープ
Extracellular. -
ポジティブ・コントロール
- Flow Cyt: Rat thymocytes.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. -
ポリ/モノ
モノクローナル -
クローン名
OX-35 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab239230の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 2 µg/ml.
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特記事項 |
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Flow Cyt
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 24932 Rat
- SwissProt: P05540 Rat
- Unigene: 10748 Rat
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別名
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
画像
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Flow cytometry analysis of rat thymocytes stained using ab239230 (concentration in sample 1 μg/ml) showing separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed).
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Flow cytometric analysis of rat thymocytes labeling CD4 with ab239230. Surface staining. Gated on thymocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab239230 は論文での使用が確認できていません。