AB30356

FITC Anti-CD32B + CD32A 抗体 [AT10]

Name: FITC Anti-CD32B + CD32A 抗体 [AT10] (ab30356) | Abcam
Brand: Abcam Limited
SKU: AB30356
Price: 35000 JPY
Availability: InStock

FITC Anti-CD32B + CD32A antibody [AT10]

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(4 Publications)

Mouse Monoclonal FCG2B antibody - conjugated to FITC. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human samples. Cited in 4 publications.

別名を表示する

CD32, FCG2, IGFR2, FCGR2B, Low affinity immunoglobulin gamma Fc region receptor II-b, IgG Fc receptor II-b, CDw32, Fc-gamma RII-b, Fc-gamma-RIIb, FcRII-b

2 Images

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

ab30356 staining CD32A + CD32B in K562 cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab30356 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in K562 cells fixed with 80% methanol (5 min).

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Overlay histogram showing K562 cells stained with ab30356 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab30356, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

AT10

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in K562 cells fixed with 4% formaldehyde (10 min) and 80% methanol (5 min).</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle|Store in the dark

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

See full target information FCGR2B

追加のターゲット

FCGR2A

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 22:87 PubMed26475492

2015

FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Shiang-Jong Tzeng,Wan-Yu Li,Hui-Ying Wang

Veterinary immunology and immunopathology 61:181-93 PubMed9613433

1998

Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

I Lilliehöök,A Johannisson,L Håkansson

Blood 91:2369-80 PubMed9516136

1998

Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene.

Applications

Unspecified application

Species

Unspecified reactive species

D Metes,L K Ernst,W H Chambers,A Sulica,R B Herberman,P A Morel

Molecular immunology 28:1243-54 PubMed1835758

1991

Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors.

Applications

Unspecified application

Species

Unspecified reactive species

J Greenman,A L Tutt,A J George,K A Pulford,G T Stevenson,M J Glennie

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD32B + CD32A 抗体 [AT10]

FITC Anti-CD32B + CD32A antibody [AT10]

Immunocytochemistry/ Immunofluorescence - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

Flow Cytometry (Intracellular) - FITC Anti-CD32B + CD32A antibody [AT10] (AB30356)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (4)

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Abcam product promise