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FITC Anti-CD3 抗体 [UCHT1] (ab34275)

Reviews (5) Submit a question References (13)
Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)
  • Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)
  • Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)

Key features and details

  • FITC Mouse monoclonal [UCHT1] to CD3
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Isotype: IgG1

製品の概要

  • 製品名

    FITC Anti-CD3 antibody [UCHT1]
    CD3 一次抗体 製品一覧

  • 製品の詳細

    FITC Mouse monoclonal [UCHT1] to CD3

  • 由来種

    Mouse

  • 標識

    FITC. Ex: 493nm, Em: 528nm

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Chimpanzee

  • 免疫原

    Tissue/ cell preparation (Human) -infant thymocytes and lymphocytes from a patient with Sezary Syndrome.

  • ポジティブ･コントロール

    • human peripheral blood lymphocytes

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab34275の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (5)
Use at an assay dependent concentration.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 
特記事項
Flow Cyt
Use at an assay dependent concentration.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

画像

  • Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)
    Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)

    Flow Cytometry analysis of Human peripheral blood lymphocytes staining CD3 with ab34275 at 5µL (1.0µg) concentration (solid line), and isotype control Mouse IgG1 (FITC) at 1.0µg (dotted line).

  • Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)
    Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)This image is courtesy of an anonymous Abreview
    ab34275 staining CD3 in Human PBMCs by Flow Cytometry. PBMCs were isolated from buffy coat after Ficoll preparation in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
    Gating Strategy: Live Lymphocytes.

    See Abreview

  • Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)
    Flow Cytometry - FITC Anti-CD3 antibody [UCHT1] (ab34275)

    FACS analysis of CD3 expression in human peripheral blood lymphocytes stained with ab34275 (solid line) at 0.25 ug and 0.25 ug isotype control (dashed line).

参考文献 (13)

ab34275 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab34275 は 13 報の論文で使用されています。

  • Ji S  et al. Cancer stem cell-derived CHI3L1 activates the MAF/CTLA4 signaling pathway to promote immune escape in triple-negative breast cancer. J Transl Med 21:721 (2023). PubMed: 37838657
  • Wen L  et al. Changes in the balance of Th17/Treg cells and oxidative stress markers in patients with HIV‑associated pulmonary tuberculosis who develop IRIS. Exp Ther Med 25:271 (2023). PubMed: 37206552
  • Dang G  et al. T lymphocyte-derived extracellular vesicles aggravate abdominal aortic aneurysm by promoting macrophage lipid peroxidation and migration via pyruvate kinase muscle isozyme 2. Redox Biol 50:102257 (2022). PubMed: 35149342
  • Lan J  et al. Cord Blood Natural Killer Cells Inhibit Sepsis Caused by Feces-Induced Acute Peritonitis via Increasing Endothelium Integrity. Cell Transplant 31:9636897221090257 (2022). PubMed: 35438589
  • Cui C  et al. Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor. BMC Immunol 23:58 (2022). PubMed: 36434505
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Flow Cytometry abreview for Anti-CD3 antibody [UCHT1] (FITC)

 Poor
Abreviews
abreview image
Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 06 2012

Flow Cytometry abreview for Anti-CD3 antibody [UCHT1] (FITC)

 Poor
Abreviews
abreview image
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 04 2012

Flow Cytometry abreview for Anti-CD3 antibody [UCHT1] (FITC)

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed and red blood cells lysed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 27 2012

Flow Cytometry abreview for Anti-CD3 antibody [UCHT1] (FITC)

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: from buffy coat after ficoll preparation
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 17 2012

Flow Cytometry abreview for Anti-CD3 antibody [UCHT1] (FITC)

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (splenocytes)
Specification
splenocytes
Preparation
Cell harvesting/tissue preparation method: after spleen mashing
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 17 2012

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