AB24844

FITC Anti-CD28 抗体 [PV-1]

Name: FITC Anti-CD28 抗体 [PV-1] (ab24844) | Abcam
Brand: Abcam Limited
SKU: AB24844
Price: 23000 JPY
Availability: InStock

FITC Anti-CD28 antibody [PV-1]

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(1 Publication)

Armenian hamster Monoclonal CD28 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human, Mouse samples. Cited in 1 publication.

別名を表示する

CD28, T-cell-specific surface glycoprotein CD28, TP44

1 Images

Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (AB24844)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (AB24844)

ab24844 at a concentration of 3 μg/10⁶ cells staining human A 20 (B cell lymphoma cells) and EL-4 cells by flow cytometry. A 50 : 50 mixture of the A20 B-cell lymphoma and the EL-4 T-cell lymphoma was stained with ab24844. Large cells were gated and analyzed by flow cytometry.

Key facts

宿主種

Armenian hamster

クローン性

Monoclonal

クローン番号

PV-1

アイソタイプ

IgG

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

交差種

Mouse, Human

アプリケーション

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18473'>ab18473</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "3 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18473'>ab18473</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

バッファー組成

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Store in the dark

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.

Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).

See full target information CD28

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of leukocyte biology 96:473-80 PubMed24935957

2014

Characterization of lung inflammation and its impact on macrophage function in aging.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia H Canan,Nandan S Gokhale,Bridget Carruthers,William P Lafuse,Larry S Schlesinger,Jordi B Torrelles,Joanne Turner

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-CD28 抗体 [PV-1]

FITC Anti-CD28 antibody [PV-1]

Flow Cytometry - FITC Anti-CD28 antibody [PV-1] (AB24844)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

標識

励起波長/蛍光波長

キャリアフリー

交差種

アプリケーション

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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組合せ製品

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Sample Prep & Detection Kits

Abcam product promise