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AB28061

FITC Anti-CD14 抗体 [MEM-15]

FITC Anti-CD14 antibody [MEM-15]

5

(6 Reviews)

|

(13 Publications)

Mouse Monoclonal CD14 antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human CD14.

別名を表示する

CD14, Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

2 Images
Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

Flow cytometry analysis of human peripheral blood cells labelling CD14 with ab28061.

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)
  • Flow Cyt

AbReview15883****

Flow Cytometry - FITC Anti-CD14 antibody [MEM-15] (AB28061)

ab28061 staining CD14 in peripheral blood mononuclear cells by flow cytometry. The fibroblasts were prepared using 10 uL FITC antiCD14 to 50 uL (~100,000) cells in PBS/5%FCS and incubated on ice for 45 min. Cells were gated on viable cells.

This image is courtesy of an anonymous Abreview

関連する標識済み抗体及び組成の異なる製品 (8)

  • 660 APC

    APC Anti-CD14 antibody [MEM-15]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD14 antibody [MEM-15]

  • 723 Alexa Fluor® 700

    Alexa Fluor® 700 Anti-CD14 antibody [MEM-15]

  • Biotin

    Biotin Anti-CD14 antibody [MEM-15]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD14 antibody [MEM-15]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD14 antibody [MEM-15]

  • 675 PerCP

    PerCP Anti-CD14 antibody [MEM-15]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD14 antibody [MEM-15]

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

MEM-15

アイソタイプ

IgG1

軽鎖のタイプ

kappa

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

Native Full Length Protein corresponding to Human CD14.

P08571

特異性

Ab28061 recognises CD14 expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. Ab28061 also recognises soluble forms of CD14 found in serum and in the urine of some nephrotic patients.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "20 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100 μl of whole blood.</p>" } } }
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製品の詳細

The antibody is free of unconjugated FITC.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: 0.2% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD14 also known as cluster of differentiation 14 is a glycoprotein with a molecular mass of approximately 55 kDa. This protein is expressed mainly on the surface of macrophages monocytes and to a lesser extent on neutrophils. CD14 exists in two forms: membrane-bound (mCD14) and soluble (sCD14). It functions as a co-receptor along with TLR4 (Toll-like receptor 4) and MD-2 for the detection of bacterial lipopolysaccharide (LPS) which is an important component of the outer membrane of Gram-negative bacteria.
Biological function summary

This glycoprotein plays a significant role in the innate immune system by recognizing pathogen-associated molecular patterns (PAMPs). It is part of a receptor complex where CD14 acts alongside LPS binding protein (LPS-R) TLR4 and MD-2 to facilitate pro-inflammatory signaling in response to microbial invasion. By binding to LPS CD14 initiates and amplifies the immune response leading to the production of cytokines and the recruitment of other immune cells to the site of infection.

Pathways

CD14 interacts with the toll-like receptor signaling pathway notably involving TLR4. Upon LPS detection CD14 assists in the activation of TLR4 triggering downstream signaling cascades such as NF-κB and MAPK pathways. These pathways result in the transcription of inflammatory cytokines and are essential for the host defense mechanism. The protein complex involving CD14 further interacts with adaptor proteins like MyD88 and TRIF facilitating signal propagation and the immune system's ability to respond to pathogens.

Researchers have linked CD14 to sepsis and atherosclerosis. High levels of soluble CD14 (sCD14) are often observed in patients with sepsis indicating its participation in excessive systemic inflammation. In atherosclerosis CD14's role in recognizing LPS contributes to chronic inflammation and the development of plaques in arteries. Through these diseases CD14 connects with TLR4 emphasizing its impact on inflammatory responses and its potential as a therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Coreceptor for bacterial lipopolysaccharide (PubMed : 1698311, PubMed : 23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed : 20133493, PubMed : 22265692, PubMed : 23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed : 8612135). Acts as a coreceptor for TLR2 : TLR6 heterodimer in response to diacylated lipopeptides and for TLR2 : TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed : 23880187).
See full target information CD14
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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e23059 PubMed38149183

2023

In vitro immunomodulatory effect of solid versus porous phosphate-based glass microspheres using macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Soumya Sheela,Fatma Mousa AlGhalban,Ifty Ahmed,Ensanya A Abou Neel

Annals of biomedical engineering 52:48-56 PubMed37989902

2023

Harnessing the Electrochemical Effects of Electroporation-Based Therapies to Enhance Anti-tumor Immune Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Zaid S Salameh,Kenneth N Aycock,Nastaran Alinezhadbalalami,Khan Mohammad Imran,Iain H McKillop,Irving C Allen,Rafael V Davalos

Journal of lipid research 64:100380 PubMed37094639

2023

Effect of type 2 diabetes on the inducible degrader of LDL receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Sum Lam,David Tak Wai Lui,Sammy Wing Ming Shiu,Ying Wong,Kathryn Choon Beng Tan

Stem cells international 2022:2164057 PubMed36311041

2022

miR-31 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Alleviates Intervertebral Disc Degeneration by Inhibiting NFAT5 and Upregulating the Wnt/-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Baodong Wang,Na Xu,Li Cao,Xiaojun Yu,Shanxi Wang,Qikun Liu,Yinguang Wang,Haoran Xu,Yang Cao

Endocrine connections 11: PubMed35560019

2022

Effects of statins on the inducible degrader of low-density lipoprotein receptor in familial hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Melody Lok-Yi Chan,Sammy Wing-Ming Shiu,Ching-Lung Cheung,Anskar Yu-Hung Leung,Kathryn Choon-Beng Tan

Cell death discovery 7:224 PubMed34455417

2021

Exosomal microRNA-15a from mesenchymal stem cells impedes hepatocellular carcinoma progression via downregulation of SALL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Shui Ma,Ji-Bin Liu,Lan Lin,Hui Zhang,Jian-Jun Wu,Yi Shi,Cheng-You Jia,Dan-Dan Zhang,Fei Yu,Hui-Min Wang,Yu-Zhen Yin,Xiao-Hui Jiang,Pei-Yao Wang,Lin-Lin Tian,Ping-Sheng Cao,Xu-Ming Wu,Hai-Min Lu,Li-Peng Gu,Jia-Jia Zhang,Gu-Jun Cong,Pei Luo,Xiao-Ming Zhong,Bo Cai,Min-Xin Shi,Su-Qing Zhang,Liu Li,Wen-Jie Zhang,Yu Liu,Zhi-Zhen Li,Ting-Miao Wu,Zhi-Jun Wu,Gao-Ren Wang,Zhong-Wei Lv,Chang-Chun Ling,Kai-Jian Chu,Da Fu

Journal of cellular and molecular medicine 25:1867-1883 PubMed33417281

2021

Human bone marrow mesenchymal stem cell-derived extracellular vesicles impede the progression of cervical cancer via the miR-144-3p/CEP55 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Meng,Baofang Zhang,Yingming Zhang,Shuyan Wang,Xiaohui Zhu

Frontiers in physiology 11:441 PubMed32528301

2020

Exosomal MicroRNA-320a Derived From Mesenchymal Stem Cells Regulates Rheumatoid Arthritis Fibroblast-Like Synoviocyte Activation by Suppressing CXCL9 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Meng,Bing Qiu

Stem cell research & therapy 8:246 PubMed29096715

2017

Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Shi,JingWei Gao,Yao Jiang,Baolan Sun,Wei Lu,Min Su,Yunzhao Xu,Xiaoqing Yang,Yuquan Zhang

Oncology letters 14:1766-1774 PubMed28789407

2017

Myeloid-derived suppressor cells are increased and correlated with type 2 immune responses, malnutrition, inflammation, and poor prognosis in patients with breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Gonda,Masahiko Shibata,Tohru Ohtake,Yoshiko Matsumoto,Kazunoshin Tachibana,Noriko Abe,Hitoshi Ohto,Kenichi Sakurai,Seiichi Takenoshita
View all publications
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