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AB24874

FITC Anti-CD11b 抗体 [M1/70]

FITC Anti-CD11b antibody [M1/70]

4

(3 Reviews)

|

(25 Publications)

Anti-CD11b antibody [M1/70] conjugated to FITC - suitable for flow cytometry, and reacts with mouse samples. Clone [M1/70] is a rat monoclonal antibody and one of the most commonly used clones for flow cytometry.

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別名を表示する

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam

1 Images
Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (AB24874)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [M1/70] (AB24874)

Flow Cytometry analysis of Mouse C57BL/6 bone marrow cells labeling CD11b with ab24874 at 0.5μg (solid line), Rat IgG2b (FITC) isotype control (dashed line) at 0.5μg concentration.

関連する標識済み抗体及び組成の異なる製品 (6)

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

M1/70

アイソタイプ

IgG2b

軽鎖のタイプ

kappa

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

交差種

Mouse

アプリケーション

Flow Cyt

applications

免疫原

Cell preparation containing Itgam protein. The exact immunogen used to generate this antibody is proprietary information.

P05555

特異性

Alpha M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer.

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代替製品

Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

  • 4
  • 1
Primary Antibodies

AB187537

Anti-CD11b antibody [EP1345Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - BSA and Azide free (AB187537)

  • 4
  • 1
Primary Antibodies

AB216445

Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (AB216445)

  • 0
  • 0
Primary Antibodies

AB52478

Anti-CD11b antibody [EP1345Y] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (AB52478)

  • 5
  • 5

組合せ製品

Assay Kits

AB277412

Human CD11b ELISA Kit

ELISA - Human CD11b ELISA Kit (AB277412)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB303467

Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Ly6g antibody [EPR22909-135] (AB303467)

  • 0
  • 0
Primary Antibodies

AB313900

Alexa Fluor® 488 Anti-CD11c antibody [KB90]

Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11c antibody [KB90] (AB313900)

  • 0
  • 0
Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/fitc-rat-igg2b-kappa-monoclonal-eb149-10h5-isotype-control-ab136125'>ab136125</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
バッファー組成
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, 0.1% Gelatin
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze|Store undiluted
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).
See full target information Itgam
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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1581878 PubMed40539039

2025

Identification of the microglia-associated signature in experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wu,Jianhong Wang,Bo Chen,Yuxue Guo,Ping Gan,Yanbing Han

Glia 73:1272-1287 PubMed39988890

2025

Generation of an Inducible Destabilized-Domain Cre Mouse Line to Target Disease Associated Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Caden M Henningfield,Minh Ngo,Kaitlin M Murray,Nellie E Kwang,Kate I Tsourmas,Jonathan Neumann,Zachary A Pashkutz,Shimako Kawauchi,Vivek Swarup,Thomas E Lane,Grant R MacGregor,Kim N Green

Acta pharmaceutica Sinica. B 14:3983-4000 PubMed39309491

2024

Heme oxygenase 1-mediated ferroptosis in Kupffer cells initiates liver injury during heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ru Li,Riqing Wei,Chenxin Liu,Keying Zhang,Sixiao He,Zhifeng Liu,Junhao Huang,Youyong Tang,Qiyuan An,Ligen Lin,Lishe Gan,Liying Zhao,Xiaoming Zou,Fudi Wang,Yuan Ping,Qiang Ma

Cellular & molecular biology letters 29:100 PubMed38977961

2024

New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Lu,Xiaojian Shi,Qiang Fu,Yaguang Han,Lei Zhu,Zhibin Zhou,Yongchuan Li,Nan Lu

Journal of neuroinflammation 21:157 PubMed38879499

2024

Cystatin F attenuates neuroinflammation and demyelination following murine coronavirus infection of the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Amber R Syage,Collin Pachow,Kaitlin M Murray,Caden Henningfield,Kellie Fernandez,Annie Du,Yuting Cheng,Gema Olivarria,Shimako Kawauchi,Grant R MacGregor,Kim N Green,Thomas E Lane

Stem cells international 2023:2759679 PubMed37576407

2023

miR-4780 Derived from N2-Like Neutrophil Exosome Aggravates Epithelial-Mesenchymal Transition and Angiogenesis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Wang,Yuqiang Shan,Sixin Zheng,Jiangtao Li,Peng Cui

International journal of biological sciences 19:1123-1145 PubMed36923940

2023

Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian Zhu

Frontiers in microbiology 13:958132 PubMed36212814

2022

Cocktail of isobavachalcone and curcumin enhance eradication of biofilm from orthopedic implants by gentamicin and alleviate inflammatory osteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Hao Hu,Fangli Huang,Zemin Ling,Bolin Chen,Bizhi Tan,Tingxuan Wang,Xiao Liu,Chun Liu,Xuenong Zou

Frontiers in chemistry 10:955995 PubMed36034656

2022

Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Shang,Juntao Sun,Xin Wu,Qinghai Wang

Frontiers in endocrinology 13:871548 PubMed35634492

2022

ICA II Alleviates Testicular Torsion Injury by Dampening the Oxidative and Inflammatory Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ani Chi,Bicheng Yang,Xiaohui Cao,Zhenqing Wang,Hanchao Liu,Hao Dai,Chunhua Deng,Min Zhang
View all publications
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