FITC Anti-CCR5 抗体 [HEK/1/85a] (ab11466)
Key features and details
- FITC Rat monoclonal [HEK/1/85a] to CCR5
- Suitable for: IHC-Fr, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
製品の概要
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製品名
FITC Anti-CCR5 antibody [HEK/1/85a]
CCR5 一次抗体 製品一覧 -
製品の詳細
FITC Rat monoclonal [HEK/1/85a] to CCR5 -
由来種
Rat -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: IHC-Fr, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Tissue, cells or virus corresponding to Human CCR5. CHO cells transfected with human CCR5
Database link: P51681 -
特記事項
This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purification by affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
HEK/1/85a -
アイソタイプ
IgG2a -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11466の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt | (5) |
Use at an assay dependent concentration.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates. -
組織特異性
Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. -
関連疾患
Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1234 Human
- Entrez Gene: 12774 Mouse
- Entrez Gene: 117029 Rat
- Omim: 601373 Human
- SwissProt: P51681 Human
- SwissProt: P51682 Mouse
- SwissProt: O08556 Rat
- Unigene: 450802 Human
see all -
別名
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab11466 は 7 報の論文で使用されています。
- Lin J et al. CCL5/CCR5-mediated peripheral inflammation exacerbates blood‒brain barrier disruption after intracerebral hemorrhage in mice. J Transl Med 21:196 (2023). PubMed: 36918921
- Elbaz-Hayoun S et al. CCR1 mediates Müller cell activation and photoreceptor cell death in macular and retinal degeneration. Elife 12:N/A (2023). PubMed: 37903056
- Li H et al. Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis. Acta Pharm Sin B 12:228-245 (2022). PubMed: 35127382
- Coomer CA et al. Single-cell glycolytic activity regulates membrane tension and HIV-1 fusion. PLoS Pathog 16:e1008359 (2020). PubMed: 32084246
- Li H et al. Inhibition of phosphodiesterase-4 attenuates murine ulcerative colitis through interference with mucosal immunity. Br J Pharmacol N/A:N/A (2019). PubMed: 30883697
- Mukherjee SP et al. Macrophage sensing of single-walled carbon nanotubes via Toll-like receptors. Sci Rep 8:1115 (2018). PubMed: 29348435
- Lee JW et al. The HIV co-receptor CCR5 regulates osteoclast function. Nat Commun 8:2226 (2017). PubMed: 29263385