FITC Anti-C1q 抗体 (ab4223)
Key features and details
- FITC Rabbit polyclonal to C1q
- Suitable for: IHC-P, Flow Cyt, ICC
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
製品の概要
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製品名
FITC Anti-C1q antibody
C1q 一次抗体 製品一覧 -
製品の詳細
FITC Rabbit polyclonal to C1q -
由来種
Rabbit -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: IHC-P, Flow Cyt, ICCmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human C1q. C1q isolated from the normal human serum.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4223の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration. PubMed: 21746929
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. PubMed: 21746929 ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
関連疾患
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻訳後修飾
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 712 Human
- Entrez Gene: 713 Human
- Entrez Gene: 714 Human
- Omim: 120550 Human
- Omim: 120570 Human
- Omim: 120575 Human
- SwissProt: P02745 Human
- SwissProt: P02746 Human
see all -
別名
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
画像
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C1q isolated from the NHS. This antibody reacts with the Human C1q complement. Traces of contaminating antibodies have been removed by solid phase absorption with human plasma proteins. This antibody is well suited for tissue section staining but may also be used for other applications.
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Immunocytochemistry - FITC Anti-C1q antibody (ab4223)Image from Phuan PW et al., J Biol Chem. 2012 Apr 20;287(17):13829-39. Epub 2012 Mar 5. Fig 7.; doi: 10.1074/jbc.M112.344325; April 20, 2012, The Journal of Biological Chemistry, 287, 13829-13839.ab4223, staining C1q in CHO-K1 cells transfected with M1-AQP4 or M23-AQP4 by Immunocytochemistry/ Immunofluorescence.
Cells were incubated at room temperature with recombinant monoclonal NMO antibody and 120 µg/ml human C1q protein (ab96363). Cells were washed with PBS, fixed in paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were blocked with 1% BSA for 30 min and incubated for 60 min with primary antibody against C1q (ab4223) at 1/200 dilution.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab4223 は 10 報の論文で使用されています。
- Izadi A et al. Subclass-switched anti-spike IgG3 oligoclonal cocktails strongly enhance Fc-mediated opsonization. Proc Natl Acad Sci U S A 120:e2217590120 (2023). PubMed: 37011197
- Li HY et al. C-Reactive Protein Protects Against Acetaminophen-Induced Liver Injury by Preventing Complement Overactivation. Cell Mol Gastroenterol Hepatol 13:289-307 (2022). PubMed: 34536564
- Esaulova E et al. The immune landscape in tuberculosis reveals populations linked to disease and latency. Cell Host Microbe 29:165-178.e8 (2021). PubMed: 33340449
- Pham A et al. Anti-LPS IgA and IgG Can Inhibit Serum Killing of Pseudomonas aeruginosa in Patients with Cystic Fibrosis. Infect Immun 89:e0041221 (2021). PubMed: 34460286
- Aoyama M et al. Effects of terminal galactose residues in mannose a1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies. MAbs 11:826-836 (2019). PubMed: 30990348
- Burbano C et al. Potential Involvement of Platelet-Derived Microparticles and Microparticles Forming Immune Complexes during Monocyte Activation in Patients with Systemic Lupus Erythematosus. Front Immunol 9:322 (2018). PubMed: 29545790
- Burbano C et al. Extracellular vesicles are associated with the systemic inflammation of patients with seropositive rheumatoid arthritis. Sci Rep 8:17917 (2018). PubMed: 30559453
- Fox S et al. The role of C1q in recognition of apoptotic epithelial cells and inflammatory cytokine production by phagocytes during Helicobacter pylori infection. J Inflamm (Lond) 12:51 (2015). PubMed: 26357509
- Phuan PW et al. Complement-dependent Cytotoxicity in Neuromyelitis Optica Requires Aquaporin-4 Protein Assembly in Orthogonal Arrays. J Biol Chem 287:13829-39 (2012). ICC/IF . PubMed: 22393049
- Barfod L et al. Evasion of immunity to Plasmodium falciparum malaria by IgM masking of protective IgG epitopes in infected erythrocyte surface-exposed PfEMP1. Proc Natl Acad Sci U S A 108:12485-90 (2011). Flow Cyt ; Human . PubMed: 21746929