FITC Anti-beta III Tubulin 抗体 [TU-20] (ab25770)
Key features and details
- FITC Mouse monoclonal [TU-20] to beta III Tubulin
- Suitable for: ICC, IHC-Fr
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
FITC Anti-beta III Tubulin antibody [TU-20]
beta III Tubulin 一次抗体 製品一覧 -
製品の詳細
FITC Mouse monoclonal [TU-20] to beta III Tubulin -
由来種
Mouse -
標識
FITC. Ex: 493nm, Em: 528nm -
特異性
This antibody recognizes the peptide sequence ESESQGPK (amino acids 441-448) of human class III beta tubulin specific for neurones. -
アプリケーション
適用あり: ICC, IHC-Frmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human beta III Tubulin aa 400-500 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Database link: Q13509 -
ポジティブ・コントロール
- ICC: Neuro2a cell line
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特記事項
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading...
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精製度
Size exclusion -
特記事項(精製)
Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
TU-20 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25770の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC |
Use a concentration of 1 µg/ml.
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IHC-Fr |
Use at an assay dependent concentration.
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特記事項 |
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ICC
Use a concentration of 1 µg/ml. |
IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. -
組織特異性
Expression is primarily restricted to central and peripheral nervous system. -
関連疾患
Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 22152 Mouse
- SwissProt: Q9ERD7 Mouse
- Unigene: 40068 Mouse
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別名
- beta 3 tubulin antibody
- beta 4 antibody
- beta-4 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab25770 は 7 報の論文で使用されています。
- Zhang S et al. Alteration of long non-coding RNAs and mRNAs expression profiles by compound heterozygous ASXL3 mutations in the mouse brain. Bioengineered 12:6935-6951 (2021). PubMed: 34559584
- Rharass T et al. Ascorbic acid alters cell fate commitment of human neural progenitors in a WNT/ß-catenin/ROS signaling dependent manner. J Biomed Sci 24:78 (2017). PubMed: 29037191
- Rharass T et al. Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/ß-catenin pathway activation to facilitate cell differentiation. J Biol Chem 289:27937-51 (2014). ICC ; Human . PubMed: 25124032
- Gilmont RR et al. Bioengineering of physiologically functional intrinsically innervated human internal anal sphincter constructs. Tissue Eng Part A 20:1603-11 (2014). PubMed: 24328537
- Lemcke H et al. Neuronal differentiation requires a biphasic modulation of gap junctional intercellular communication caused by dynamic changes of connexin43 expression. Eur J Neurosci 38:2218-28 (2013). PubMed: 23607708
- Raghavan S et al. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141:310-9 (2011). IHC-Fr ; Mouse . PubMed: 21463628
- Launay S et al. HtrA1-dependent proteolysis of TGF-beta controls both neuronal maturation and developmental survival. Cell Death Differ 15:1408-16 (2008). IHC-FoFr ; Mouse . PubMed: 18551132