JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB3554

FITC Anti-6X His tag® 抗体

FITC Anti-6X His tag® antibody

5

(1 Review)

|

(1 Publication)

Chicken Polyclonal 6X His tag® antibody - conjugated to FITC. Suitable for ELISA, WB and reacts with Tag samples. Cited in 1 publication.

別名を表示する

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

Key facts

宿主種

Chicken

クローン性

Polyclonal

アイソタイプ

IgY

軽鎖のタイプ

unknown

標識

FITC

励起波長/蛍光波長

Ex: 495nm, Em: 519nm

キャリアフリー

No

アプリケーション

WB, ELISA

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>Prior to conjugation, the antibodies were analyzed by ELISA.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Prior to conjugation, the antibodies were analyzed by Western Blot.</p>" } } }
style
left-column
style
left-column

製品の詳細

Molar F/P ratio is 3.6:1.

HIS-TAG® is a trademark of EMD Biosciences, Inc.

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7 Preservative: 0.1% Sodium azide Constituents: 0.2% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.
Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

See full target information 6X His tag®
style
left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 180:323-339.e19 PubMed31928845

2020

Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Del Toro,Maria A Carrasquero-Ordaz,Amy Chu,Tobias Ruff,Meriam Shahin,Verity A Jackson,Matthieu Chavent,Miguel Berbeira-Santana,Goenuel Seyit-Bremer,Sara Brignani,Rainer Kaufmann,Edward Lowe,Rüdiger Klein,Elena Seiradake
View all publications
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB5000

Anti-6X His tag® antibody [4D11]

primary-antibodies

6x-his-tag-antibody-4d11-ab5000

4

(4 reviews)

Alternative Product
Primary Antibodies

AB202004

HRP Anti-6X His tag® antibody [4D11]

primary-antibodies

hrp-6x-his-tag-antibody-4d11-ab202004

0

(0 reviews)

Alternative Product
Primary Antibodies

AB245203

Anti-6X His tag® antibody [JPAR-2] - BSA and Azide free

primary-antibodies

6x-his-tag-antibody-jpar-2-bsa-and-azide-free-ab245203

0

(0 reviews)

Alternative Product
Primary Antibodies

AB213204

Anti-6X His tag® antibody [EPR20547] - ChIP Grade

primary-antibodies

6x-his-tag-antibody-epr20547-chip-grade-ab213204

1

(1 reviews)

Alternative Product
Primary Antibodies

AB245114

Anti-6X His tag® antibody [JPAR-2]

primary-antibodies

6x-his-tag-antibody-jpar-2-ab245114

0

(0 reviews)

Alternative Product
Primary Antibodies

AB232492

Anti-6X His tag® antibody [EPR20547] - BSA and Azide free

primary-antibodies

6x-his-tag-antibody-epr20547-bsa-and-azide-free-ab232492

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com