FITC Anti-6X His tag® 抗体 (ab1206)
Key features and details
- FITC Rabbit polyclonal to 6X His tag®
- Suitable for: ELISA, Flow Cyt
- Reacts with: Species independent
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
製品の概要
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製品名
FITC Anti-6X His tag® antibody
6X His tag® 一次抗体 製品一覧 -
製品の詳細
FITC Rabbit polyclonal to 6X His tag® -
由来種
Rabbit -
標識
FITC. Ex: 493nm, Em: 528nm -
アプリケーション
適用あり: ELISA, Flow Cytmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to 6X His tag® conjugated to keyhole limpet haemocyanin.
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特記事項
FITC/Protein Ratio = 3.0 – 6.0.
HIS-TAG® is a trademark of EMD Biosciences, Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to fluorescein isothiocyanate. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1206の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt | (5) |
Use at an assay dependent concentration.
ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab37406 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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関連性
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells. -
細胞内局在
Depends upon the localization of the parent protein tagged with hexahistidine. -
別名
- 6 His epitope tag antibody
- Hexa His tag antibody
- HHHHHH epitope tag antibody
see all
画像
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Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)Li et al PLoS One. 2018 Jan 22;13(1):e0191024. doi: 10.1371/journal.pone.0191024. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Muc1-Bi-1 and Muc1-Bi-2 can recognize Muc1 positive tumor cells.
a). Flow cytometry analysis of Muc1-Bi-1 and Muc1-Bi-2 on Muc1-negative cell lines HePG2 and CHO and Muc1-positive cell lines, HT29. Red line indicates cells with no staining; green line indicates cells with only anti-His-FITC staining (ab1206); blue line indicates cells with positive control anti-Muc1 antibody; purple line indicates cells with Muc1-Bi-1 protein; orange line indicates cells with Muc1-Bi-2 protein.
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Flow Cytometry - FITC Anti-6X His tag® antibody (ab1206)This image is courtesy of an anonymous Abreviewab1206 staining 6X His tag® in human endometrial carcinoma cells by Flow Cytometry.
Cells were prepared by short trypsinisation in PBS/1% FBS/0.2% sodium azide and fixed with formaldehyde. The gate included all single cells. The sample was incubated with a 6X His tag® primary antibody for 1 hour at 4°C. ab1206 was used as the secondary. On the image, the red line represents cells stained with ab1206 alone, and the blue line is the tested sample carrying a 6XHis tag®. The small peak to the right represents cells that bound the tested sample.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (29)
ab1206 は 29 報の論文で使用されています。
- Merikhian P et al. Recombinant nanobody against MUC1 tandem repeats inhibits growth, invasion, metastasis, and vascularization of spontaneous mouse mammary tumors. Mol Oncol 16:485-507 (2022). PubMed: 34694686
- Jeong BS et al. Computational design of a neutralizing antibody with picomolar binding affinity for all concerning SARS-CoV-2 variants. MAbs 14:2021601 (2022). PubMed: 35030983
- Hudák A et al. Syndecan-4 Is a Key Facilitator of the SARS-CoV-2 Delta Variant's Superior Transmission. Int J Mol Sci 23:N/A (2022). PubMed: 35054983
- Liu W et al. HVEM structures and mutants reveal distinct functions of binding to LIGHT and BTLA/CD160. J Exp Med 218:N/A (2021). PubMed: 34709351
- Shan Z et al. Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment. Elife 10:N/A (2021). PubMed: 34110284