AB16466

Anti-Firefly Luciferase 抗体 [Luci17]

Name: Anti-Firefly Luciferase 抗体 [Luci17] (ab16466) | Abcam
Brand: Abcam Limited
SKU: AB16466
Rating: 4 (5 reviews)

Anti-Firefly Luciferase antibody [Luci17]

(5 Reviews)

(22 Publications)

Mouse Monoclonal LUCI antibody. Suitable for WB and reacts with Transfected cell lysate - Firefly samples. Cited in 22 publications. Immunogen corresponding to Recombinant Fragment Protein within Firefly LUCI_PHOPY.

別名を表示する

Luciferin 4-monooxygenase, Luciferase

1 Images

Lab

Western blot - Anti-Firefly Luciferase antibody [Luci17] (AB16466)

Lanes 1 - 4 : Merged signal (red and green). Green – ab16466 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab16466 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1 : 10000 dilution for 1hr at room temperature and then imaged.

All lanes:

Western blot - Anti-Firefly Luciferase antibody [Luci17] (ab16466) at 1 µg/mL

Lane 1:

Non-transfected 293 whole cell lysate at 20 µg

Lane 2:

Firefly Luciferase transfected 293 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

NIH3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/10000 dilution

Observed band size: 65 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

Luci17

アイソタイプ

IgG1

軽鎖のタイプ

unknown

キャリアフリー

交差種

Firefly

アプリケーション

applications

免疫原

Recombinant Fragment Protein within Firefly LUCI_PHOPY. The exact immunogen used to generate this antibody is proprietary information.

P08659

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Firefly": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Firefly": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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下記製品にもご興味をお持ちいただけるかもしれません:

AB228530

Luciferase Reporter Assay Substrate Kit - Firefly

0 Reviews

製品ページを見る

こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

バッファー組成

pH: 7.2 Preservative: 0.08% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Firefly luciferase also known as luc assay or luciferase protein is an enzyme that catalyzes the oxidation of luciferin resulting in bioluminescence. This protein has a molecular mass of approximately 61 kDa and is responsible for the characteristic glow of fireflies. The luciferase expression occurs mainly in the light-producing organs of fireflies such as in the insect Photinus pyralis where it plays an important role in light emission.

Biological function summary

Firefly luciferase converts chemical energy into light making it valuable for various biological applications as a reporter gene. It participates in the formation of a complex with its substrates luciferin and ATP resulting in the production of a photon. This enzyme is widely used in molecular and cellular biology experiments to monitor gene expression and study protein-protein interactions due to its luminescent properties.

Pathways

Firefly luciferase functions in the bioluminescent pathway involving the conversion of luciferin to oxyluciferin and light emission. It interfaces with ATP a universal energy carrier which highlights its integration into the cellular energy systems. Firefly luciferase is similar to other oxidative enzymes though it is not directly involved with metabolic pathways like the TCA cycle or glycolysis.

Researchers often utilize firefly luciferase as a tool in tumor studies or infectious disease models. Its luminescence aids in imaging and tracking biological processes in real-time. While firefly luciferase itself is not directly associated with specific diseases the use of firefly agents allows researchers to study disease outcomes assess therapeutic efficacy and understand gene expression profiles related to cancer and viral infections often in connection with proteins such as p53 in cancer research.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Produces green light with a wavelength of 562 nm.

See full target information LUCI_PHOPY

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文献 (22)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 17:10407-10422 PubMed37120837

2023

Bioengineered Bacteriophage-Like Nanoparticles as RNAi Therapeutics to Enhance Radiotherapy against Glioblastomas.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Han Pang,Chiung-Yin Huang,Pin-Yuan Chen,Nan-Si Li,Ying-Pei Hsu,Jan-Kai Wu,Hsiu-Fang Fan,Kuo-Chen Wei,Hung-Wei Yang

Molecular therapy. Methods & clinical development 27:352-367 PubMed36381301

2022

Intrabiliary infusion of naked DNA vectors targets periportal hepatocytes in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sereina Deplazes,Andrea Schlegel,Zhuolun Song,Gabriella Allegri,Nicole Rimann,Tanja Scherer,Melanie Willimann,Lennart Opitz,Sharon C Cunningham,Ian E Alexander,Anja Kipar,Johannes Häberle,Beat Thöny,Hiu Man Grisch-Chan

Cell death & disease 13:158 PubMed35177591

2022

Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zihang Ling,Wei Li,Jiaqi Hu,Yuanyuan Li,Miao Deng,Siyuan Zhang,Xianyue Ren,Tong Wu,Juan Xia,Bin Cheng,Xiaoan Tao

Nature communications 12:4789 PubMed34373451

2021

Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Athea Vichas,Amanda K Riley,Naomi T Nkinsi,Shriya Kamlapurkar,Phoebe C R Parrish,April Lo,Fujiko Duke,Jennifer Chen,Iris Fung,Jacqueline Watson,Matthew Rees,Austin M Gabel,James D Thomas,Robert K Bradley,John K Lee,Emily M Hatch,Marina K Baine,Natasha Rekhtman,Marc Ladanyi,Federica Piccioni,Alice H Berger

Nature communications 12:2041 PubMed33824314

2021

Bioengineered bacteria-derived outer membrane vesicles as a versatile antigen display platform for tumor vaccination via Plug-and-Display technology.

Applications

Unspecified application

Species

Unspecified reactive species

Keman Cheng,Ruifang Zhao,Yao Li,Yingqiu Qi,Yazhou Wang,Yinlong Zhang,Hao Qin,Yuting Qin,Long Chen,Chen Li,Jie Liang,Yujing Li,Jiaqi Xu,Xuexiang Han,Gregory J Anderson,Jian Shi,Lei Ren,Xiao Zhao,Guangjun Nie

Molecular therapy oncolytics 17:278-292 PubMed32368616

2020

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne A Hammill,Jacek M Kwiecien,Anna Dvorkin-Gheva,Vivian W C Lau,Christopher Baker,Ying Wu,Ksenia Bezverbnaya,Craig Aarts,Christopher W Heslen,Galina F Denisova,Heather Derocher,Katy Milne,Brad H Nelson,Jonathan L Bramson

Nutrients 12: PubMed32121454

2020

Anti-stress, Glial- and Neuro-differentiation Potential of Resveratrol: Characterization by Cellular, Biochemical and Imaging Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Sajal Afzal,Sukant Garg,Divya Adiga,Yoshiyuki Ishida,Keiji Terao,Sunil C Kaul,Renu Wadhwa

Marine drugs 17: PubMed30909572

2019

Rat Glioma Cell-Based Functional Characterization of Anti-Stress and Protein Deaggregation Activities in the Marine Carotenoids, Astaxanthin and Fucoxanthin.

Applications

Unspecified application

Species

Unspecified reactive species

Sajal Afzal,Sukant Garg,Yoshiyuki Ishida,Keiji Terao,Sunil C Kaul,Renu Wadhwa

The Journal of biological chemistry 293:17646-17660 PubMed30242124

2018

The microRNA-23a cluster regulates the developmental HoxA cluster function during osteoblast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Tanner C Godfrey,Benjamin J Wildman,Marcio M Beloti,Austin G Kemper,Emanuela P Ferraz,Bhaskar Roy,Mohammad Rehan,Lubana H Afreen,Eddy Kim,Christopher J Lengner,Quamarul Hassan

Nature communications 9:450 PubMed29386504

2018

Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Schmid,Patrick Ernst,Annemarie Honegger,Maarit Suomalainen,Martina Zimmermann,Lukas Braun,Sarah Stauffer,Cristian Thom,Birgit Dreier,Matthias Eibauer,Anja Kipar,Viola Vogel,Urs F Greber,Ohad Medalia,Andreas Plückthun

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Anti-Firefly Luciferase 抗体 [Luci17]

Anti-Firefly Luciferase antibody [Luci17]

Western blot - Anti-Firefly Luciferase antibody [Luci17] (AB16466)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

下記製品にもご興味をお持ちいただけるかもしれません:

Luciferase Reporter Assay Substrate Kit - Firefly

こちらの製品も推奨する理由

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (22)

Abcam product promise

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