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リコンビナントRabMAb

Anti-Firefly Luciferase 抗体 [EPR17789] - N-terminal (ab185923)

Submit a review Submit a question References (8)
Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
  • Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
  • Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
  • Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
  • Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
  • Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17789] to Firefly Luciferase - N-terminal
  • Suitable for: ICC/IF, WB, Flow Cyt
  • Reacts with: Firefly

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

製品の概要

  • 製品名

    Anti-Firefly Luciferase antibody [EPR17789] - N-terminal
    Firefly Luciferase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17789] to Firefly Luciferase - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, Flow Cytmore details

  • 種交差性

    交差種: Firefly
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Firefly Luciferase transfected HEK-293 whole cell lysate. ICC/IF: Firefly Luciferase transfected HEK-293T cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR17789

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185923の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/500.
WB
1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Flow Cyt
Use at an assay dependent concentration.
特記事項
ICC/IF
1/500.
WB
1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Flow Cyt
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.

  • 細胞内局在

    Peroxisome

  • 参照データベース

    • 別名

      • ec 1.13.12.7 antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      Immunocytochemistry/ Immunofluorescence - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (Human epithelial cells from embryonic kidney) cells transfected with Empty vector or Firefly Luciferase, labeling Firefly Luciferase with ab185923 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HEK293T cells transfected with Firefly Luciferase is observed. The nuclear counter stain is DAPI (blue). 

    • Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      Flow Cytometry - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

      Flow Cytometry analysis of 293T (Human epithelial cell line from embryonic kidney) transfected with GFP tagged Firefly Luciferase cells labeling Firefly Luciferase with purified ab185923 at 1/200 dilution (10ug/ml, Right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 647)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Left panel) was used as the isotype control.

    • Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/2000 dilution

      Lane 1 : Empty vector (vector control) transfected HEK293 (human embryonic kidney) whole cell lysate
      Lane 2 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 30 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/1000 dilution

      Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate at 10 µg
      Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate at 20 µg
      Lane 3 : MCF-7 (human breast carcinoma) whole cell lysate at 20 µg
      Lane 4 : Jurkat (human acute T cell leukemia) whole cell lysate at 20 µg
      Lane 5 : SH-SY5Y (human neuroblastoma) whole cell lysate at 20 µg
      Lane 6 : Human fetal heart lysate at 10 µg
      Lane 7 : Human fetal kidney lysate at 10 µg
      Lane 8 : Human fetal spleen lysate at 10 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      Western blot - Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      All lanes : Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923) at 1/1000 dilution

      Lane 1 : Firefly Luciferase transfected HEK293 (human embryonic kidney) whole cell lysate
      Lane 2 : Mouse brain lysate
      Lane 3 : Mouse heart lysate
      Lane 4 : Mouse kidney lysate
      Lane 5 : Mouse spleen lysate
      Lane 6 : Rat brain lysate
      Lane 7 : Rat kidney lysate
      Lane 8 : Rat spleen lysate
      Lane 9 : C6 (rat glioma) whole cell lysate lysate
      Lane 10 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
      Lane 11 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 12 : NIH/3T3 (mouse embryo) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 61 kDa
      Observed band size: 61 kDa


      Exposure time: 3 minutes


      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)
      Anti-Firefly Luciferase antibody [EPR17789] - N-terminal (ab185923)

    参考文献 (8)

    ab185923 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185923 は 8 報の論文で使用されています。

    • Isobe S  et al. Reduced FOXF1 links unrepaired DNA damage to pulmonary arterial hypertension. Nat Commun 14:7578 (2023). PubMed: 37989727
    • Svitkin YV  et al. Membrane-dependent relief of translation elongation arrest on pseudouridine- and N1-methyl-pseudouridine-modified mRNAs. Nucleic Acids Res 50:7202-7215 (2022). PubMed: 34933339
    • Zhang J  et al. Epithelial Gasdermin D shapes the host-microbial interface by driving mucus layer formation. Sci Immunol 7:eabk2092 (2022). PubMed: 35119941
    • Dong X  et al. Variation around the dominant viral genome sequence contributes to viral load and outcome in patients with Ebola virus disease. Genome Biol 21:238 (2020). PubMed: 32894206
    • Azad T  et al. A LATS biosensor screen identifies VEGFR as a regulator of the Hippo pathway in angiogenesis. Nat Commun 9:1061 (2018). WB . PubMed: 29535383
    • Yan J  et al. Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter. PeerJ 6:e5701 (2018). PubMed: 30386688
    • Svitkin YV  et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2a-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Res 45:6023-6036 (2017). PubMed: 28334758
    • García-Dorival I  et al. Elucidation of the Cellular Interactome of Ebola Virus Nucleoprotein and Identification of Therapeutic Targets. J Proteome Res 15:4290-4303 (2016). PubMed: 27786485

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