Anti-Firefly Luciferase 抗体 (ab181640)
Key features and details
- Goat polyclonal to Firefly Luciferase
- Suitable for: WB
- Reacts with: Firefly
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Firefly Luciferase antibody
Firefly Luciferase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Firefly Luciferase -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Firefly -
免疫原
Full length native protein (purified) corresponding to Firefly (Photinus pyralis) Firefly Luciferase aa 1-550.
Sequence:MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVN ITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV AVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKII IMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSG STGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMF TTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTL IDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAIL ITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVD YVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL
Database link: P08659 -
ポジティブ･コントロール
- Luciferase protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
ab181640 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. ab181640 is sterile filtered. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181640の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 61 kDa.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 61 kDa. |
ターゲット情報
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関連性
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. -
細胞内局在
Peroxisome -
参照データベース
- SwissProt: P08659 Firefly
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別名
- ec 1.13.12.7 antibody
- Firefly antibody
- Luciferase antibody
- Luciferin 4 monooxygenase antibody
画像
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Western blot - Anti-Firefly Luciferase antibody (ab181640)All lanes : Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution
Lane 1 : Non-transfected 293 cell lysate
Lane 2 : Firefly Luciferase transfected 293 cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : NIH3T3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green – ab181640 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab181640 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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Western blot - Anti-Firefly Luciferase antibody (ab181640)Anti-Firefly Luciferase antibody (ab181640) at 1/1000 dilution + Luciferase protein at 0.05 µg
Secondary
Peroxidase anti-goat secondary antibody at 1/40000 dilution
Predicted band size: 61 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab181640 は 15 報の論文で使用されています。
- Hamester F et al. Insights into the Steps of Breast Cancer-Brain Metastases Development: Tumor Cell Interactions with the Blood-Brain Barrier. Int J Mol Sci 23:N/A (2022). PubMed: 35163822
- Allen V et al. VIVA1: a more invasive subclone of MDA-MB-134VI invasive lobular carcinoma cells with increased metastatic potential in xenograft models. Br J Cancer 127:56-68 (2022). PubMed: 35318435
- Troiani M et al. Single-cell transcriptomics identifies Mcl-1 as a target for senolytic therapy in cancer. Nat Commun 13:2177 (2022). PubMed: 35449130
- Freytag V et al. Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice. Methods Mol Biol 2524:317-325 (2022). PubMed: 35821483
- Shao H et al. Converting melanoma-associated fibroblasts into a tumor-suppressive phenotype by increasing intracellular Notch1 pathway activity. PLoS One 16:e0248260 (2021). PubMed: 33705467