AB81468

Anti-Filaggrin 抗体

Name: Anti-Filaggrin 抗体 (ab81468) | Abcam
Brand: Abcam Limited
SKU: AB81468
Rating: 4 (9 reviews)

Anti-Filaggrin antibody

(9 Reviews)

(35 Publications)

Anti-Filaggrin antibody (ab81468) is a rabbit polyclonal antibody detecting Filaggrin in ICC/IF. Suitable for Human.

- Over 30 publications
- Trusted since 2009

別名を表示する

Filaggrin, FLG

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (AB81468)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (AB81468)

ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF

applications

免疫原

Synthetic Peptide within Human FLG. The exact immunogen used to generate this antibody is proprietary information.

P20930

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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製品の詳細

What is this antibody validated in?
Anti-Filaggrin antibody (ab81468) is a rabbit polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-Filaggrin (ab81468) was first used in a scientific publication in 2009 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-Filaggrin (ab81468) has over 5 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

See full target information FLG

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文献 (35)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:22357 PubMed38102220

2023

β-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hyung-Su Kim,Hyoung-June Kim,Yong-Deog Hong,Eui Dong Son,Si-Young Cho

Nature communications 14:7660 PubMed37996412

2023

Vector-borne Trypanosoma brucei parasites develop in artificial human skin and persist as skin tissue forms.

Applications

Unspecified application

Species

Unspecified reactive species

Christian Reuter,Laura Hauf,Fabian Imdahl,Rituparno Sen,Ehsan Vafadarnejad,Philipp Fey,Tamara Finger,Nicola G Jones,Heike Walles,Lars Barquist,Antoine-Emmanuel Saliba,Florian Groeber-Becker,Markus Engstler

Frontiers in immunology 14:1273182 PubMed38053996

2023

Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yuan Wang,Yu-Xin Zheng,Fan Xu,Ying-Zhe Cui,Xue-Yan Chen,Si-Qi Chen,Bing-Xi Yan,Yuan Zhou,Min Zheng,Xiao-Yong Man

International journal of molecular sciences 24: PubMed38003573

2023

Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human Keratinocyte Models.

Applications

Unspecified application

Species

Unspecified reactive species

Jamie M L Kok,Georgina C Dowd,Jaydee D Cabral,Lyn M Wise

Journal of nanobiotechnology 21:354 PubMed37775761

2023

Bioinspired polydopamine nanoparticles as efficient antioxidative and anti-inflammatory enhancers against UV-induced skin damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Zhang,Yuqi Zhou,Zhaoting Jiang,Chenhui He,Bo Wang,Qi Wang,Zeqian Wang,Tong Wu,Xiaoqi Chen,Ziwei Deng,Chunying Li,Zhe Jian

International journal of molecular sciences 24: PubMed37762646

2023

Epicutaneous Sensitization to the Phytocannabinoid β-Caryophyllene Induces Pruritic Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Saadet Inan,Sara J Ward,Citlalli T Baltazar,Gabrielle A Peruggia,Elham Javed,Ajay P Nayak

International journal of bioprinting 9:738 PubMed37323493

2023

Developing a bioink for single-step deposition and maturation of human epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth Jinfen Chai,Wan Ling Wong,Cyrus Weijie Beh

STAR protocols 3:101613 PubMed35990735

2022

Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gowher Ali,Essam M Abdelalim

Cells 11: PubMed35741029

2022

Alpha-Keratin, Keratin-Associated Proteins and Transglutaminase 1 Are Present in the Ortho- and Parakeratinized Epithelium of the Avian Tongue.

Applications

Unspecified application

Species

Unspecified reactive species

Kinga Skieresz-Szewczyk,Hanna Jackowiak,Marek Skrzypski

International journal of molecular sciences 22: PubMed34884594

2021

Development of an Aged Full-Thickness Skin Model Using Flexible Skin-on-a-Chip Subjected to Mechanical Stimulus Reflecting the Circadian Rhythm.

Applications

Unspecified application

Species

Unspecified reactive species

Subin Jeong,Jisue Kim,Hye Mi Jeon,Kyunghee Kim,Gun Yong Sung

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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Anti-Filaggrin 抗体

Anti-Filaggrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Filaggrin antibody (AB81468)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (35)

Abcam product promise

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