Anti-FIGNL1 抗体 (ab70841)
Key features and details
- Rabbit polyclonal to FIGNL1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FIGNL1 antibody
FIGNL1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FIGNL1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
-
免疫原
Synthetic peptide within Human FIGNL1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q6PIW4 -
ポジティブ･コントロール
- Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70841の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).
|
|
| IP |
Use at 2-5 µg/mg of lysate.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa). |
|
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
-
関連性
May regulate osteoblast proliferation and differentiation. -
参照データベース
- Entrez Gene: 63979 Human
- Entrez Gene: 60530 Mouse
- SwissProt: Q6PIW4 Human
- SwissProt: Q8BPY9 Mouse
-
別名
- Fidgetin like protein 1 antibody
画像
-
Western blot - Anti-FIGNL1 antibody (ab70841)All lanes : Anti-FIGNL1 antibody (ab70841) at 1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 74 kDa
Observed band size: 71 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FIGNL1 antibody (ab70841)Detection of Human FIGNL1 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded), using ab70841 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using 1 µg/mg ab70841 and chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab70841 は論文での使用が確認できていません。