Anti-Fibulin 5 抗体 [1G6A4] (ab66339)
![Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)](https://www.abcam.co.jp/ps/products/66/ab66339/Images/ab66339-210121-ab66339wb.jpg "Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)")
Key features and details
- Mouse monoclonal [1G6A4] to Fibulin 5
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-Fibulin 5 antibody [1G6A4]
Fibulin 5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1G6A4] to Fibulin 5 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant His tagged fragment, corresponding to amino acids 242-448 of Human Fibulin 5
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ポジティブ･コントロール
- Colon and breast cancer
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR171104-21 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
1G6A4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/500 - 1/2000. Predicted molecular weight: 50 kDa.
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| IHC-P |
1/500 - 1/2000.
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| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Predicted molecular weight: 50 kDa. |
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IHC-P
1/500 - 1/2000. |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling. -
組織特異性
Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes. -
関連疾患
Defects in FBLN5 are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. Autosomal dominant cutis laxa is a relatively benign inherited and acquired connective tissue disorder.
Defects in FBLN5 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3) [MIM:608895]. ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. -
配列類似性
Belongs to the fibulin family.
Contains 6 EGF-like domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 10516 Human
- Omim: 604580 Human
- SwissProt: Q9UBX5 Human
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別名
- ADCL2 antibody
- ARCL1A antibody
- ARMD3 antibody
see all
画像
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Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)This image is courtesy of an anonymous AbreviewAnti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution + Human vascular smooth muscle whole cell lysate at 20 µg
Secondary
HRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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![Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)](https://www.abcam.co.jp/ps/products/66/ab66339/Images/fb1.jpg)
Western blot - Anti-Fibulin 5 antibody [1G6A4] (ab66339)Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution + Immunogen (recombinant truncated protein) at 1 µg
Predicted band size: 50 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)](https://www.abcam.co.jp/ps/products/66/ab66339/Images/fb2a.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)Immunohistochemical analysis of paraffin embedded human colon cancer tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)](https://www.abcam.co.jp/ps/products/66/ab66339/Images/fb2b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibulin 5 antibody [1G6A4] (ab66339)Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (ab66339)](https://www.abcam.co.jp/ps/products/66/ab66339/Images/Fibulin-5-Primary-antibodies-ab66339-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Fibulin 5 antibody [1G6A4] (ab66339)ICC/IF image of ab66339 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66339, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab66339 は 17 報の論文で使用されています。
- Chen T et al. Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway. Br J Cancer 129:1261-1273 (2023). PubMed: 37644092
- Yan Y et al. Effect of fibulin-5 on aldosterone-induced apoptosis in human ascending aortic smooth muscle cells. Exp Ther Med 22:896 (2021). PubMed: 34257709
- Chakraborty M et al. Primary Human Trabecular Meshwork Model for Pseudoexfoliation. Cells 10:N/A (2021). PubMed: 34943956
- Li R et al. FBLN5 is targeted by microRNA-27a-3p and suppresses tumorigenesis and progression in high-grade serous ovarian carcinoma. Oncol Rep 44:2143-2151 (2020). PubMed: 32901854
- O'Neill Moore S et al. Insights into the biophysical forces between proteins involved in elastic fiber assembly. J Mater Chem B 8:9239-9250 (2020). PubMed: 32966543