Name: Anti-Fibronectin antibody [F1]
Brand: RabMAb
SKU: ab32419
Rating: 5 (7 reviews)

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Western blot - Anti-Fibronectin antibody [F1] (ab32419)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [F1] to Fibronectin
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free FITC

製品名

Anti-Fibronectin antibody [F1]
Fibronectin 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [F1] to Fibronectin
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
種交差性

交差種: Human
免疫原

Recombinant full length protein within Human Fibronectin aa 1-2400. The exact sequence is proprietary.
Database link: P02751
ポジティブ･コントロール
- WB: Human serum and plasma. SK-OV-3 and HepG2 cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HepG2 and human mesenchymal stem cells. Flow Cyt (intra): HepG2 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

F1
アイソタイプ

IgG
研究分野

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Fibronectin type III EDB protein (ab209886)
Related Products
- Native Mouse Fibronectin protein (ab92784)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab32419の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/20.
WB	(3)	1/1000. Predicted molecular weight: 263 kDa.
IHC-P	(2)	1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF	(1)	1/150.

特記事項
Flow Cyt (Intra) 1/20.
WB 1/1000. Predicted molecular weight: 263 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/150.

機能

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
組織特異性

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
関連疾患

Glomerulopathy with fibronectin deposits 2
配列類似性

Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains.
発生段階

Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
翻訳後修飾

Sulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
細胞内局在

Secreted, extracellular space, extracellular matrix.
Target information above from: UniProt accession P02751 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
別名
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
- DKFZp686F10164 antibody
- DKFZp686H0342 antibody
- DKFZp686I1370 antibody
- DKFZp686O13149 antibody
- ED B antibody
- Fibronectin 1 antibody
- FINC antibody
- FINC_HUMAN antibody
- FN antibody
- FN1 antibody
- FNZ antibody
- GFND antibody
- GFND2 antibody
- LETS antibody
- Migration stimulating factor antibody
- MSF antibody
- Ugl-Y3 antibody
see all

Western blot - Anti-Fibronectin antibody [F1] (ab32419)

All lanes : Anti-Fibronectin antibody [F1] (ab32419) at 1/5000 dilution (purified)

Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : SK-OV-3 (Human ovarian cancer cell line) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 263 kDa
Observed band size: 263 kDa

Blocking/Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [F1] (ab32419)

Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab32419 at a dilution of 1/250. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)

ICC/IF image of unpurified ab32419 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells. The cells were fixed in 4% formaldehyde (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32419 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight^® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

Alexa Fluor^® 488 (ab198933) and Alexa Fluor^® 647 (ab198934) conjugated versions are available for this clone.
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (ab32419)This image is courtesy of an anonymous abreview.

ICC/IF image of unpurified ab32419 stained human mesenchymal stem cells. The cells were fixed in paraformaldehyde and then incubated in 0.1%BSA / 1% goat serum for 30 minutes, to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32419, 1/100 dilution) for 2 hours at 22°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-rabbit IgG. DAPI was used to stain the cell nuclei (blue).
See Abreview
Flow Cytometry (Intracellular) - Anti-Fibronectin antibody [F1] (ab32419)

Intracellular Flow Cytometry analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Fibronectin with purified ab32419 at 1/20 dilution (10µg/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Western blot - Anti-Fibronectin antibody [F1] (ab32419)

Anti-Fibronectin antibody [F1] (ab32419) at 1/1000 dilution (purified) + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 263 kDa
Observed band size: 263 kDa

Blocking/Dilution buffer: 5% NFDM/TBST
Anti-Fibronectin antibody [F1] (ab32419)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab32419 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32419 は 105 報の論文で使用されています。

Wang Q et al. Construction of a HOXA11-AS-Interact Ed Network in Keloid Fibroblasts Using Integrated Bioinformatic Analysis and in Vitro Validation. Front Genet 13:844198 (2022). PubMed: 35432479
Zhang S & Chen R LINC01140 regulates osteosarcoma proliferation and invasion by targeting the miR-139-5p/HOXA9 axis. Biochem Biophys Rep 31:101301 (2022). PubMed: 35800618
Yang C et al. Comprehensive analysis of the prognostic value and immune infiltration of FGFR family members in gastric cancer. Front Oncol 12:936952 (2022). PubMed: 36147913
Mou K et al. Zwint facilitates melanoma progression by promoting c-Myc expression. Exp Ther Med 22:818 (2021). PubMed: 34131441
Wang J et al. Anti-Fibrotic Effects of Low Toxic Microcystin-RR on Bleomycin-Induced Pulmonary Fibrosis: A Comparison with Microcystin-LR. Front Pharmacol 12:675907 (2021). PubMed: 34168562

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-10 of 10 Abreviews or Q&A

Application

Western blot

Sample

Human Tissue lysate - whole (Hepatic organoids)

Gel Running Conditions

Reduced Denaturing (10 % gel)

Loading amount

20 µg

Specification

Hepatic organoids

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 19 2021

Application

IHC - Wholemount

Sample

Human Tissue (Hepatic organoids)

Specification

Hepatic organoids

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 16 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Skin)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 08 2017

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (gel 8%)

Sample

Human Cell lysate - whole cell (ovarian cancer cell line: SKOV3)

Specification

ovarian cancer cell line: SKOV3

Blocking step

Blocking one (nacalai tesque) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 27°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 18 2013

Application

Western blot

Sample

Human Cell lysate - whole cell (Bone marrow MSCs)

Loading amount

15 µg

Specification

Bone marrow MSCs

Gel Running Conditions

Reduced Denaturing (10% gel)

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 11 2012

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Bone marrow MSCs)

Specification

Bone marrow MSCs

Fixative

Paraformaldehyde

Permeabilization

Blocking step

0.1% BSA + 1% goat serum as blocking agent for 30 minute(s) · Concentration: 1%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 11 2012

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (lung, cystic fibrosis)

Specification

lung, cystic fibrosis

Fixative

Formaldehyde

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA, 100C, 20 minutes

Permeabilization

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 02 2009

Question

we are looking into buying two antibody against human fibronectin and elastin for paraffin blocks (IHC). We need both antibodies to not react against rat or bovine fibronectin or elastin.

Abcam community

Verified customer

Asked on Nov 29 2013

Answer

Fibronectin is a highly conserved protein and the full human protein shares 93% homology with cow and 91% homology with rat therefore there is a large chance that an anti-human antibody will also cross react with cow and rat. In addition, there is no stretch of amino acids in the human protein which is very different from the cow and rat sequences so I think it will be extremely hard to find an antibody specific to human only and for this reason, we do not carry such an antibody in our catalogue.

Although elastin is less conserved between species, all of our anti-elastin antibodies tested in IHC have either been tested in rat and/or cow and have been shown to cross-react or have simply not been tested so we cannot guarantee the cross-reactivity. Unfortunately, for the antibodies which haven't been tested in cow or rat, we have not determined the epitope and so cannot perform sequence alignments to predict whether the antibody is likely to recognise cow and rat and so I am unable to suggest an antibody which may be suitable for your needs.

Elisa Thomas

Abcam Scientific Support

Answered on Nov 29 2013

Question

Sehr geehrte Damen und Herren, als Verwenderin Ihres Antikörpers C5b-9(ab 55811) bin ich auf der Suche nach einem Fibronectin Antikörper zur Detektion von Infarkten in humanem Myocard in der IHC auf Paraffinschnitten. Bitte empfehlen Sie mir geeignete Antikörper aus Ihrer Produktpalette mit den entsprechenden Preisen, gerne per Email an o.g. Adresse oder auch per Fax zur Weiterleitung an mich.

Abcam community

Verified customer

Asked on Jul 26 2012

Answer

Vielen Dank für Ihre Anfrage.

Wir haben da mehrere Antikörper, die für Sie in Frage kommen könnten. Ich habe mir erlaubt, zwei Antikörper herauszusuchen, die ich für geeignet halte:

Click here (or use the following: https://www.abcam.com/Fibronectin-antibody-F1-ab32419.html).

Click here (or use the following: https://www.abcam.com/Fibronectin-antibody-IST-9-ab6328.html).

ab6328 ist sehr gut etabliert, und demonstrierte gute Färbung im Infarkt, allerdings im Kaninchen. Desweiteren finden sich gute Kundenrezessionen und viele Publikationen für diesen Antikörper.

Bei ab32419 handelt es sich um einen monoklonalen Antikörper aus dem Kaninchen. Diese Antikörper zeichnen sich durch eine sehr Gosse und spezifische Zielprotein- Affinität aus. Desweiteren zeigt gute Färbung und ist die günstigere Alternative.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Benutzen Sie unsere Produkte? Schicken Sie uns einen Abreview. Verdienen Sie sich eine Belohnung!
https://www.abcam.com/abreviews

Abcam Scientific Support

Answered on Jul 26 2012

Question

Does this antibody recognize fibronection form A or B?

Abcam community

Verified customer

Asked on May 26 2006

Answer

Thank you for your enquiry. If you refer to form A or B as to alternative splice variants known as ED-A and ED-B (ED stands for extra domain), these alternative splice forms are expressed in a cell-type specific manner. We used plasma fibronectin, which is made in the liver, as an immunogen. Plasma fibronectin does not contain ED-A or ED-B; these extra domains are only found in so-called "cellular fibronectin" which is present in tissues. Therefore, it is very unlikely that our antibodies would distinguish between the different variants. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on May 31 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

発生段階

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (105)

レビューと Q&A

Western blot abreview for Anti-Fibronectin antibody [F1]

IHC - Wholemount abreview for Anti-Fibronectin antibody [F1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fibronectin antibody [F1]

Western blot abreview for Anti-Fibronectin antibody [F1]

Western blot abreview for Anti-Fibronectin antibody [F1]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Fibronectin antibody [F1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Fibronectin antibody [F1]