Anti-Fibrinogen 抗体 (ab27913)
Key features and details
- Rabbit polyclonal to Fibrinogen
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Fibrinogen antibody
Fibrinogen 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Fibrinogen -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISA, IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Full length native protein (purified): Mouse fibrinogen isolated from plasma using several chromatographic steps.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27913の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
組織特異性
Plasma. -
関連疾患
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
ドメイン
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻訳後修飾
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 110135 Mouse
- Entrez Gene: 14161 Mouse
- Entrez Gene: 99571 Mouse
- SwissProt: Q8K0E8 Mouse
- SwissProt: Q8VCM7 Mouse
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別名
- FGA antibody
- FGB antibody
- FGG antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (10)
ab27913 は 10 報の論文で使用されています。
- Shi C et al. Neutrophil circadian rhythm is associated with different outcomes of acute kidney injury due to cholesterol crystal embolism. Front Cardiovasc Med 9:974759 (2022). PubMed: 35966530
- Li FJ et al. Fibrinogen mediates cadmium-induced macrophage activation and serves as a predictor of cadmium exposure in chronic obstructive pulmonary disease. Am J Physiol Lung Cell Mol Physiol 322:L593-L606 (2022). PubMed: 35200041
- Kerr BA et al. Platelet TSP-1 controls prostate cancer-induced osteoclast differentiation and bone marrow-derived cell mobilization through TGFß-1. Am J Clin Exp Urol 9:18-31 (2021). PubMed: 33816691
- Hulmi JJ et al. Muscle NAD+ depletion and Serpina3n as molecular determinants of murine cancer cachexia-the effects of blocking myostatin and activins. Mol Metab 41:101046 (2020). PubMed: 32599075
- Li X et al. Deficiency of Mouse FHR-1 Homolog, FHR-E, Accelerates Sepsis, and Acute Kidney Injury Through Enhancing the LPS-Induced Alternative Complement Pathway. Front Immunol 11:1123 (2020). PubMed: 32636836
- Uyama N et al. Anti-interleukin-6 receptor antibody treatment ameliorates postoperative adhesion formation. Sci Rep 9:17558 (2019). PubMed: 31772282
- Masuda H et al. Batroxobin accelerated tissue repair via neutrophil extracellular trap regulation and defibrinogenation in a murine ischemic hindlimb model. PLoS One 14:e0220898 (2019). PubMed: 31419236
- Wan H et al. Role of von Willebrand factor and ADAMTS-13 in early brain injury after experimental subarachnoid hemorrhage. J Thromb Haemost 16:1413-1422 (2018). PubMed: 29729651
- Zhang Y et al. Inverse agonist of ERR? reduces cannabinoid receptor type 1-mediated induction of fibrinogen synthesis in mice with a high-fat diet-intoxicated liver. Arch Toxicol 92:2885-2896 (2018). PubMed: 30019168
- Nordstrom SM et al. Increased thrombosis susceptibility and altered fibrin formation in STAT5-deficient mice. Blood 116:5724-33 (2010). WB ; Mouse . PubMed: 20823455
