AB154806

Anti-Fibrillarin + Fibrillarin-like protein 1 抗体 [EPR10822(B)] - Nucleolar Marker

Name: Anti-Fibrillarin 抗体 [EPR10822(B)] - Nucleolar Marker (ab154806) | Abcam
Brand: Abcam Limited
SKU: AB154806
Price: 35000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker

RabMAb
Recombinant
Lab Essentials
詳細を見る

(1 Review)

(6 Publications)

Rabbit Recombinant Monoclonal Fibrillarin antibody. Nucleolus marker. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

別名を表示する

FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin, RNA 2'-O-methyltransferase FBLL1, Fibrillarin like 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

Immunofluorescence analysis of HepG2 cells labeling Fibrillarin with ab154806 at 1/250 dilution.

Unknown

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

All lanes:

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (ab154806) at 1/1000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

HepG2 lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Lane 4:

Molt4 lysate at 10 µg

Predicted band size: 33 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR10822(B)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed : 24352239, PubMed : 30540930, PubMed : 32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed : 30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed : 32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed : 32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed : 24352239, PubMed : 30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information FBL

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6606 PubMed40675952

2025

Somatostatin-expressing interneurons induce early NO-driven and late specific astrocyte-mediated vasodilation.

Applications

Unspecified application

Species

Unspecified reactive species

Thanh Tan Vo,Won Beom Jung,Tong Jin,Geun Ho Im,Soohyun Lee,Seong-Gi Kim

PLoS genetics 19:e1010854 PubMed37639467

2023

rRNA transcription is integral to phase separation and maintenance of nucleolar structure.

Applications

Unspecified application

Species

Unspecified reactive species

Soma Dash,Maureen C Lamb,Jeffrey J Lange,Mary C McKinney,Dai Tsuchiya,Fengli Guo,Xia Zhao,Timothy J Corbin,MaryEllen Kirkman,Kym Delventhal,Emma L Moore,Sean McKinney,Rita Shiang,Paul A Trainor

Molecular oncology 17:27-36 PubMed36370117

2022

Ribosome biogenesis-based predictive biomarkers in endocrine therapy (Anastrozole) combined with mTOR inhibitor (Vistusertib) in endometrial cancer: translational study from the VICTORIA trial in collaboration with the GINECO group.

Applications

Unspecified application

Species

Unspecified reactive species

Nour-El-Houda Mourksi,Cécile Dalban,Amélie Colombe-Vermorel,Laetitia Odeyer,Valentin Simioni,Jean-Sébastien Frenel,Michel Fabbro,Fernando Bazan,Sophie Abadie-Lacourtoisie,Elodie Coquan,Séverine Martinez,Gwenaelle Garin,Séverine Tabone-Eglinger,Isabelle Treilleux,Sylvie Chabaud,David Pérol,Isabelle Ray-Coquard,Pierre-Etienne Heudel,Jean-Jacques Diaz,Virginie Marcel

Bioengineering (Basel, Switzerland) 9: PubMed36004921

2022

Bioinformatic Analysis and In Vitro and In Vivo Experiments Reveal That Fibrillarin Participates in the Promotion of Lung Metastasis in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weixin Luo,Shusheng Lin,Yipei Huang,Ke Zhu,Fapeng Zhang,Junlong Lin,Yufei Qin,Ziyu Zhou,Wenrui Wu,Chao Liu

Non-coding RNA research 7:55-65 PubMed35261930

2022

Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy M F Steinbusch,Guus G H van den Akker,Andy Cremers,Adhiambo M A Witlox,Heleen M Staal,Mandy J Peffers,Lodewijk W van Rhijn,Marjolein M J Caron,Tim J M Welting

Physiological reports 5: PubMed29122955

2017

DNPEP is not the only peptidase that produces SPAK fragments in kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Rainelli Koumangoye,Eric Delpire

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrillarin + Fibrillarin-like protein 1 抗体 [EPR10822(B)] - Nucleolar Marker

Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

Western blot - Anti-Fibrillarin + Fibrillarin-like protein 1 antibody [EPR10822(B)] - Nucleolar Marker (AB154806)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

製品プロトコール

ターゲットの情報

文献 (6)

Abcam product promise