AB218846

Anti-Fibrillarin 抗体 [4A4]

Anti-Fibrillarin antibody [4A4]

(2 Reviews)

(2 Publications)

Mouse Monoclonal Fibrillarin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FBL.

別名を表示する

FIB1, FLRN, FBL, rRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase, U6 snRNA 2'-O-methyltransferase fibrillarin

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

C6 (rat glial tumor cell line) (Panel A) and HEK-293 (human epithelial cell line from embryonic kidney) (Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.

Supplier Data

Western blot - Anti-Fibrillarin antibody [4A4] (AB218846)

Fibrillarin detection is shown in green.

Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).

All lanes:

Western blot - Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution

Lane 1:

MW ladder

Lane 2:

Rat whole brain lysate

Lane 3:

Mouse whole brain lysate

Lane 4:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 5:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

Lane 6:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 7:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

Predicted band size: 33 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

4A4

アイソタイプ

IgG1

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human FBL.

P22087

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000 - 1/2000", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

バッファー組成

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins (PubMed : 24352239, PubMed : 30540930, PubMed : 32017898). Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed : 30540930). Site specificity is provided by a guide RNA that base pairs with the substrate (By similarity). Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA (By similarity). Probably catalyzes 2'-O-methylation of U6 snRNAs in box C/D RNP complexes (PubMed : 32017898). U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity (PubMed : 32017898). Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed : 24352239, PubMed : 30540930). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed : 34516797).

See full target information FBL

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:13176-13193 PubMed37988198

2023

Investigating EGF and PAG1 as necroptosis-related biomarkers for diabetic nephropathy: an and validation study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejun Wang,Linlin Zhang,Zhiping Peng

Parasitology 148:341-353 PubMed33100232

2020

infections induce G cell cycle arrest and a senescence-like phenotype in endothelial host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zahady D Velásquez,Sara López-Osorio,Daniel Waiger,Carolina Manosalva,Learta Pervizaj-Oruqaj,Susanne Herold,Carlos Hermosilla,Anja Taubert

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fibrillarin 抗体 [4A4]

Anti-Fibrillarin antibody [4A4]

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

Immunocytochemistry/ Immunofluorescence - Anti-Fibrillarin antibody [4A4] (AB218846)

Western blot - Anti-Fibrillarin antibody [4A4] (AB218846)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (2)

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Abcam product promise