Anti-FHL2 抗体 - C-terminal (ab153816)
Key features and details
- Rabbit polyclonal to FHL2 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FHL2 antibody - C-terminal
FHL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FHL2 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Synthetic peptide corresponding to a region within C-terminal amino acids 217-279 of Human FHL2 (Q14192).
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ポジティブ･コントロール
- A431, HeLa, HepG2, MOLT4, Raji whole cell lysates, Human colon carcinoma tissue, HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153816の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (1) |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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| IHC-P |
1/100 - 1/1000.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. -
組織特異性
Expressed in skeletal muscle and heart. -
配列類似性
Contains 4 LIM zinc-binding domains. -
ドメイン
The third LIM zinc-binding mediates interaction with E4F1. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 510008 Cow
- Entrez Gene: 2274 Human
- Entrez Gene: 14200 Mouse
- Entrez Gene: 63839 Rat
- Omim: 602633 Human
- SwissProt: Q2KI95 Cow
- SwissProt: Q14192 Human
- SwissProt: O70433 Mouse
see all -
別名
- AAG 11 antibody
- AAG11 antibody
- Aging associated gene 11 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-FHL2 antibody - C-terminal (ab153816)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FHL2 with ab153816 at 1/200 dilution. Image at the bottom merged with DNA probe.
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Western blot - Anti-FHL2 antibody - C-terminal (ab153816)Anti-FHL2 antibody - C-terminal (ab153816) at 1/1000 dilution + HepG2 whole cell
lysate at 30 µg
Predicted band size: 32 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FHL2 antibody - C-terminal (ab153816)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling FHL2 with ab153816 antibody at 1/500.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab153816 は論文での使用が確認できていません。