Anti-FHL2 抗体 (ab12327)
Key features and details
- Rabbit polyclonal to FHL2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FHL2 antibody
FHL2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FHL2 -
由来種
Rabbit -
特異性
Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
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ポジティブ･コントロール
- WB: U-2 OS and HeLa cell lysates; HeLa nuclear extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody is affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12327の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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| 特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
ターゲット情報
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機能
May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. -
組織特異性
Expressed in skeletal muscle and heart. -
配列類似性
Contains 4 LIM zinc-binding domains. -
ドメイン
The third LIM zinc-binding mediates interaction with E4F1. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2274 Human
- Omim: 602633 Human
- SwissProt: Q14192 Human
- Unigene: 443687 Human
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別名
- AAG 11 antibody
- AAG11 antibody
- Aging associated gene 11 antibody
see all
画像
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Western blot - Anti-FHL2 antibody (ab12327)All lanes : Anti-FHL2 antibody (ab12327) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : FHL2 knockout U-2 OS cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 32 kDaLanes 1 - 3: Merged signal (red and green). Green - ab12327 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab12327 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab12327 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-FHL2 antibody (ab12327)Samples:
Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3µ
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab12327 は 13 報の論文で使用されています。
- Jehanno C et al. Synergistic activation of genes promoting invasiveness by dual deprivation in oxygen and nutrients. Int J Exp Pathol 104:64-75 (2023). PubMed: 36694990
- Du F et al. E3 Ubiquitin Ligase Midline 1 Regulates Endothelial Cell ICAM-1 Expression and Neutrophil Adhesion in Abdominal Sepsis. Int J Mol Sci 24:N/A (2022). PubMed: 36614145
- Wan Y et al. Screening and identification of a novel FHL2 mutation by whole exome sequencing in twins with familial Waldenström macroglobulinemia. Cancer 127:2039-2048 (2021). PubMed: 33764527
- Algaber A et al. Targeting FHL2-E-cadherin axis by miR-340-5p attenuates colon cancer cell migration and invasion. Oncol Lett 22:637 (2021). PubMed: 34295384
- Kong L & Zhang C LncRNA DLX6-AS1 aggravates the development of ovarian cancer via modulating FHL2 by sponging miR-195-5p. Cancer Cell Int 20:370 (2020). PubMed: 32774164