Anti-FHL2 抗体

• WB

Lab

Western blot - Anti-FHL2 antibody (AB12327)

Lanes 1 - 3 : Merged signal (red and green). Green - ab12327 observed at 32 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab12327 was shown to react with FHL2 in wild-type U-2 OS cells in western blot with loss of signal observed in FHL2 knockout sample. Wild-type and FHL2 knockout U-2 OS cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab12327 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FHL2 antibody (ab12327) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 40 µg

Lane 2:

FHL2 knockout U-2 OS cell lysate at 40 µg

Lane 3:

HeLa cell lysate at 40 µg

Predicted band size: 32 kDa

Observed band size: 32 kDa

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• WB

Unknown

Western blot - Anti-FHL2 antibody (AB12327)

Samples : Nuclear extract (NE) from HeLa cells. Antibody : Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 μg/ml. Detection : Chemiluminescence with an exposure time of 5 minutes.

Samples : Nuclear extract (NE) from HeLa cells. Antibody : Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection : Chemiluminescence with an exposure time of 5 minutes.

All lanes:

Western blot - Anti-FHL2 antibody (ab12327)

Predicted band size: 32 kDa

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