Anti-FH/Fumarase 抗体 (ab113963)
Key features and details
- Goat polyclonal to FH/Fumarase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FH/Fumarase antibody
FH/Fumarase 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to FH/Fumarase -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Horse, Chicken, Cow, Dog, Pig, Zebrafish, Chinese hamster
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免疫原
Synthetic peptide corresponding to Human FH/Fumarase aa 150-250 (internal sequence) (Cysteine residue).
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ポジティブ･コントロール
- Human Heart and Human Skeletal Muscle tissue Human Kidney lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113963の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 55 kDa.
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| IHC-P |
Use a concentration of 3.75 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.01 µg/ml. Predicted molecular weight: 55 kDa. |
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IHC-P
Use a concentration of 3.75 µg/ml. |
ターゲット情報
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機能
Also acts as a tumor suppressor. -
パスウェイ
Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. -
関連疾患
Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839]. -
配列類似性
Belongs to the class-II fumarase/aspartase family. Fumarase subfamily. -
細胞内局在
Cytoplasm and Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 520260 Cow
- Entrez Gene: 480092 Dog
- Entrez Gene: 2271 Human
- Entrez Gene: 14194 Mouse
- Entrez Gene: 100627128 Pig
- Entrez Gene: 24368 Rat
- Entrez Gene: 393938 Zebrafish
- Omim: 136850 Human
see all -
別名
- FH antibody
- Fumarase antibody
- Fumarate hydratase antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab113963)Immunohistochemical staining of Human Heart using ab113963 at 3.75µg/ml
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FH/Fumarase antibody (ab113963)Immunohistochemical staining of Human Skeletal Muscle using ab113963 at 3.75µg/ml -
Western blot - Anti-FH/Fumarase antibody (ab113963)Anti-FH/Fumarase antibody (ab113963) at 0.01 µg/ml + Human Kidney lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 55 kDa
Primary incubation was 1 hour.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab113963 は 3 報の論文で使用されています。
- Zecchini V et al. Fumarate induces vesicular release of mtDNA to drive innate immunity. Nature 615:499-506 (2023). PubMed: 36890229
- Jiang Y et al. Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation. Nat Cell Biol 17:1158-68 (2015). WB ; Human . PubMed: 26237645
- Zecchini V et al. Nuclear ARRB1 induces pseudohypoxia and cellular metabolism reprogramming in prostate cancer. EMBO J 33:1365-82 (2014). WB, ICC/IF . PubMed: 24837709