AB133644

Anti-FGFR3 抗体 [EPR2304(3)]

Name: Anti-FGFR3 抗体 [EPR2304(3)] (ab133644) | Abcam
Brand: Abcam Limited
SKU: AB133644
Price: 32000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-FGFR3 antibody [EPR2304(3)]

RabMAb
Recombinant
KO Validated
20ul selling size
詳細を見る

(2 Reviews)

(25 Publications)

Anti-FGFR3 antibody [EPR2304(3)] (ab133644) is a rabbit monoclonal antibody detecting FGFR3 in Western Blot. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

別名を表示する

CD333, JTK4, FGFR3, Fibroblast growth factor receptor 3, FGFR-3

3 Images

Unknown

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (ab133644) at 1/2000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lane 3:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 4:

IM-9 (Human multiple myeloma B Lymphoblast) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 88 kDa

false

Lab

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

Lanes 1 - 3 : Merged signal (red and green). Green - ab133644 observed at 125kDa. Red - loading control, ab9484, observed at 37 kDa.

ab133644 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in FGFR3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FGFR3 knockout samples were subjected to SDS-PAGE. ab133644 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (ab133644) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

FGFR3 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human FGFR3 knockout HEK-293 cell line (<a href='/products/cell-lines/human-fgfr3-knockout-hek-293-cell-line-ab259775'>ab259775</a>)

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 76 kDa,88 kDa

Observed band size: 125 kDa

false

OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2304(3)

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

We have tested this antibody in positive Mouse and Rat tissue lysates in WB and could not obtain a specific band for FGFR3. Please get in touch with our Scientific Support team if you wish to share any data regarding this antibody's cross-reactivity in Mouse and Rat species.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "Abcam recommends to block in 3% non-fat milk.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-FGFR3 antibody [EPR2304(3)] (ab133644) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of FGFR3?
Anti-FGFR3 [EPR2304(3)] (ab133644) specifically detects a band for FGFR3 (UniProt: P22607) at a molecular weight of 88kDa.

Trusted by the scientific community
Anti-FGFR3 [EPR2304(3)] (ab133644) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-FGFR3 antibody [EPR2304(3)] (ab133644) has been confirmed by Immunocytochemistry testing in FGFR3 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR2304(3)] also available for your convenience: ab133644, Carrier free - ab187341

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FGFR3 or fibroblast growth factor receptor 3 is a tyrosine kinase receptor with a molecular mass of approximately 95 kDa. This receptor also known as CD333 primarily interacts with fibroblast growth factors. It resides on the cell surface and is predominately expressed in bone cartilage and the brain. FGFR3 plays a major role in cell growth regulation and differentiation affecting how cells respond to external signals.

Biological function summary

FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.

Pathways

FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.

FGFR3 mutations have significant links to skeletal disorders most notably achondroplasia characterized by short limbs and abnormal bone growth. FGFR3 also associates with bladder cancer where overactivity of the receptor leads to increased cell proliferation and survival. Mutations in FGFR3 often correlate with these conditions impacting signaling pathways and interacting with proteins such as PTPN11 highlighting its critical role in pathogenesis.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation and apoptosis. Plays an essential role in the regulation of chondrocyte differentiation, proliferation and apoptosis, and is required for normal skeleton development. Regulates both osteogenesis and postnatal bone mineralization by osteoblasts. Promotes apoptosis in chondrocytes, but can also promote cancer cell proliferation. Required for normal development of the inner ear. Phosphorylates PLCG1, CBL and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Plays a role in the regulation of vitamin D metabolism. Mutations that lead to constitutive kinase activation or impair normal FGFR3 maturation, internalization and degradation lead to aberrant signaling. Over-expressed or constitutively activated FGFR3 promotes activation of PTPN11/SHP2, STAT1, STAT5A and STAT5B. Secreted isoform 3 retains its capacity to bind FGF1 and FGF2 and hence may interfere with FGF signaling.

See full target information FGFR3

style

left-column

文献 (25)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0327588 PubMed40788860

2025

The FGFR inhibitor pemigatinib overcomes cancer drug resistance to KRAS G12C inhibitors in mesenchymal lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Abdollahi,Margaret Favata,Michael Weber,Valerie Roman,Rodrigo Hess,Kayla Hammond,Matthew R Farren,Mike Schaffer,Aidan Gilmartin,Hui Wang,Jonathan Rios-Doria,Alejandro Amador-Arjona

Cell death & disease 16:485 PubMed40603902

2025

FGFR inhibitors promote the autophagic degradation of IFN-γ-induced PD-L1 and alleviate the PD-L1-mediated transcriptional suppression of FGFR3-TACC3 in non-muscle-invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Chen Lin,Cheng-Ying Chu,Tsung-Han Hsieh,Bo-Jyun Lin,Jing-Ping Liou,Yun Yen,Chun-Han Chen

Nature communications 16:4128 PubMed40319089

2025

Harnessing the FGFR2/NF2/YAP signaling-dependent necroptosis to develop an FGFR2/IL-8 dual blockade therapeutic strategy.

Applications

Unspecified application

Species

Unspecified reactive species

Dongshao Chen,Zitong Zhao,Ruoxi Hong,Di Yang,Ying Gong,Qingnan Wu,Yan Wang,Yiren Cao,Jie Chen,Yidi Tai,Haoyu Liu,Jinting Li,Jiawen Fan,Weimin Zhang,Yongmei Song,Qimin Zhan

Journal of Cancer 15:5415-5424 PubMed39247610

2024

STAT3 inhibitor Stattic Exhibits the Synergistic Effect with FGFRs Inhibitor Erdafitinib in FGFR1-positive Lung Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongqin Zhong,Ling Wang,Xue Zhu,Shu Li,Xiyue Li,Chen Ding,Ke Wang,Xun Wang

Cell death & disease 15:279 PubMed38637504

2024

Cancer-associated fibroblasts secrete FGF5 to inhibit ferroptosis to decrease cisplatin sensitivity in nasopharyngeal carcinoma through binding to FGFR2.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Liu,Ling Tang,Huai Liu,Yanzhu Chen,Tengfei Xiao,Wangning Gu,Hongmin Yang,Hui Wang,Pan Chen

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:1200-1208 PubMed37946482

2023

Bo's abdominal acupuncture improves disordered metabolism in obese type 2 diabetic rats through regulating fibroblast growth factor 21 and its related adipokines.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xihui,Pang Jianli,Xiong Guan,Feng Jie

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23085 PubMed37462502

2023

DNMT3B activates FGFR3-mediated endoplasmic reticulum stress by regulating PTPN2 promoter methylation to promote the development of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhang,Quan Guo,Zhenzhou Zhao,Ming Nie,Qingbo Shi,En Li,Kaiyuan Liu,Haosen Yu,Lixin Rao,Muwei Li

Cancer discovery 13:1998-2011 PubMed37377403

2023

Resistance to Selective FGFR Inhibitors in FGFR-Driven Urothelial Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Facchinetti,Antoine Hollebecque,Floriane Braye,Damien Vasseur,Yoann Pradat,Rastislav Bahleda,Cédric Pobel,Ludovic Bigot,Olivier Déas,Juan David Florez Arango,Giorgia Guaitoli,Hayato Mizuta,David Combarel,Lambros Tselikas,Stefan Michiels,Sergey I Nikolaev,Jean-Yves Scoazec,Santiago Ponce-Aix,Benjamin Besse,Ken A Olaussen,Yohann Loriot,Luc Friboulet

Nature 618:862-870 PubMed37286607

2023

Structural basis for FGF hormone signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfeng Chen,Lili Fu,Jingchuan Sun,Zhiqiang Huang,Mingzhen Fang,Allen Zinkle,Xin Liu,Junliang Lu,Zixiang Pan,Yang Wang,Guang Liang,Xiaokun Li,Gaozhi Chen,Moosa Mohammadi

Cancer cell international 22:295 PubMed36167542

2022

METTL3 suppresses anlotinib sensitivity by regulating mA modification of FGFR3 in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Shuai Li,Zhexun Huang,Congyuan Cao,Anxun Wang,Qianting He

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FGFR3 抗体 [EPR2304(3)]

Anti-FGFR3 antibody [EPR2304(3)]

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

Western blot - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

OI-RD Scanning - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (25)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise