Anti-FGFR2 抗体 [EPR24075-418] - BSA and Azide free (ab289993)
![Western blot - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-483598-anti-fgfr2-antibody-epr24075418-western-blot-chronic.jpg "Western blot - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24075-418] to FGFR2 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, Flow Cyt, IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free
FGFR2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24075-418] to FGFR2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, Flow Cyt, IP, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HAP1 and KATA III whole cell lysates. IHC-P: Human breast carcinoma and gastric adenocarcinoma tissue; KATO III cell pellet. ICC/IF: KATO III cells. Flow Cyt: KATO III cells. IP: KATO III whole cell lysate.
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特記事項
ab289993 is the carrier-free version of ab289968.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24075-418 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289993の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 85, 145 kDa (predicted molecular weight: 92 kDa).
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| 特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 85, 145 kDa (predicted molecular weight: 92 kDa). |
ターゲット情報
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機能
Receptor for acidic and basic fibroblast growth factors. -
関連疾患
Defects in FGFR2 are the cause of Crouzon syndrome (CS) [MIM:123500]; also called craniofacial dysostosis type I (CFD1). CS is an autosomal dominant syndrome characterized by craniosynostosis (premature fusion of the skull sutures), hypertelorism, exophthalmos and external strabismus, parrot-beaked nose, short upper lip, hypoplastic maxilla, and a relative mandibular prognathism.
Defects in FGFR2 are a cause of Jackson-Weiss syndrome (JWS) [MIM:123150]. JWS is an autosomal dominant craniosynostosis syndrome characterized by craniofacial abnormalities and abnormality of the feet: broad great toes with medial deviation and tarsal-metatarsal coalescence.
Defects in FGFR2 are a cause of Apert syndrome (APRS) [MIM:101200]; also known as acrocephalosyndactyly type 1 (ACS1). APRS is a syndrome characterized by facio-cranio-synostosis, osseous and membranous syndactyly of the four extremities, and midface hypoplasia. The craniosynostosis is bicoronal and results in acrocephaly of brachysphenocephalic type. Syndactyly of the fingers and toes may be total (mitten hands and sock feet) or partial affecting the second, third, and fourth digits. Intellectual deficit is frequent and often severe, usually being associated with cerebral malformations.
Defects in FGFR2 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly. Three subtypes of Pfeiffer syndrome have been described: mild autosomal dominant form (type 1); cloverleaf skull, elbow ankylosis, early death, sporadic (type 2); craniosynostosis, early demise, sporadic (type 3).
Defects in FGFR2 are the cause of Beare-Stevenson cutis gyrata syndrome (BSCGS) [MIM:123790]. BSCGS is an autosomal dominant condition is characterized by the furrowed skin disorder of cutis gyrata, acanthosis nigricans, craniosynostosis, craniofacial dysmorphism, digital anomalies, umbilical and anogenital abnormalities and early death.
Defects in FGFR2 are the cause of familial scaphocephaly syndrome (FSPC) [MIM:609579]; also known as scaphocephaly with maxillary retrusion and mental retardation. FSPC is an autosomal dominant craniosynostosis syndrome characterized by scaphocephaly, macrocephaly, hypertelorism, maxillary retrusion, and mild intellectual disability. Scaphocephaly is the most common of the craniosynostosis conditions and is characterized by a long, narrow head. It is due to premature fusion of the sagittal suture or from external deformation.
Defects in FGFR2 are a cause of lacrimo-auriculo-dento-digital syndrome (LADDS) [MIM:149730]; also known as Levy-Hollister syndrome. LADDS is a form of ectodermal dysplasia, a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. LADDS is an autosomal dominant syndrome characterized by aplastic/hypoplastic lacrimal and salivary glands and ducts, cup-shaped ears, hearing loss, hypodontia and enamel hypoplasia, and distal limb segments anomalies. In addition to these cardinal features, facial dysmorphism, malformations of the kidney and respiratory system and abnormal genitalia have been reported. Craniosynostosis and severe syndactyly are not observed.
Defects in FGFR2 are the cause of Antley-Bixler syndrome (ABS) [MIM:207410]. ABS is a multiple congenital anomaly syndrome characterized by craniosynostosis, radiohumeral synostosis, midface hypoplasia, malformed ears, arachnodactyly and multiple joint contractures. ABS is a heterogeneous disorder and occurs with and without abnormal genitalia in both sexes. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2263 Human
- Omim: 176943 Human
- SwissProt: P21802 Human
- Unigene: 533683 Human
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別名
- bacteria-expressed kinase antibody
- BBDS antibody
- BEK antibody
see all
画像
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Western blot - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)All lanes : Anti-FGFR2 antibody [EPR24075-418] (ab289968) at 1/1000 dilution
Lane 1 : Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate
Lane 2 : FGFR2 KO HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 92 kDa
Observed band size: 85, 145 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab289968, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, ab289968 was shown to bind specifically to FGFR2. Two bands were observed at 145/85 kDa in wild-type HAP1 cell lysates with no signal observed at this size in FGFR2 knockout cell lysates.
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![Western blot - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-483599-anti-fgfr2-antibody-epr24075418-western-blot-gastric.jpg)
Western blot - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)All lanes : Anti-FGFR2 antibody [EPR24075-418] (ab289968) at 1/1000 dilution
Lane 1 : KATO III (human gastric carcinoma) whole cell lysate
Lane 2 : SW620 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 92 kDa
Observed band size: 85, 145 kDa why is the actual band size different from the predicted?This data was developed using ab289968, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The antibody recognizes endogenous levels of total FGFR2 protein and a C-terminal truncated form K-sam (PMID:17505008).
Negative control: SW620 (PMID: 24968263)
Exposure time: 10 seconds
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![Immunocytochemistry/ Immunofluorescence - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-8-anti-fgfr2-antibody-epr24075418-immunocytochemistry-kato-iii.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)This data was developed using ab289968, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KATO III cells labeling FGFR2 with ab289968 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing membrane staining in the KATO III cell line, while no staining was observed in the SW620 cell line. Negative control: SW620 (PMID: 24968263). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-5-anti-fgfr2-antibody-epr24075418-immunohistochemistry-human-breast-carcino.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)This data was developed using ab289968, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling FGFR2 with ab289968 at 1/100 dilution, followed by LeicaDS9800 (Bond™, Polymer Refine Detection). The section was incubated with ab289968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-6-anti-fgfr2-antibody-epr24075418-immunohistochemistry-human-gastric-adenoc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)This data was developed using ab289968, the same antibody clone in a different buffer formµlation.
Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labelling FGFR2 with ab289968 at 1/100 dilution, followed LeicaDS9800 (Bond™, Polymer Refine Detection). Membraneous staining on human gastric adenocarcinoma (PMID: 24518603). The section was incubated with ab289968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-7-anti-fgfr2-antibody-epr24075418-immunohistochemistry-a-kato-iii-cells.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)This data was developed using ab289968, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) KATO III cells labeling FGFR2 with ab289968 at 1/100 dilution followed by LeicaDS9800 (Bond™, Polymer Refine Detection). Positive staining on (A) KATO III cells. Negative control: no staining on (B) SW620 cells (PMID: 24968263). The section was incubated with ab289968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™, Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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![Immunoprecipitation - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-4-anti-fgfr2-antibody-epr24075418-immunoprecipitation-kato-iii.jpg)
Immunoprecipitation - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)This data was developed using ab289968, the same antibody clone in a different buffer formµlation.
FGFR2 was immunoprecipitated from 0.35 mg KATO III (human gastric carcinoma) whole cell lysate 10 µg with ab289968 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289968 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.Lane 1: KATO III (human gastric carcinoma) whole cell lysate 10 µg
Lane 2: ab289968 IP in KATO III whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab289968 in KATO III whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5.5 seconds
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![Flow Cytometry - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-9-anti-fgfr2-antibody-epr24075418-flow-cytometry-sw620.jpg)
Flow Cytometry - Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)This data was developed using ab289968, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of SW620 (human colorectal adenocarcinoma epithelial cell, Left) / KATO III (human gastric carcinoma, Right) cells labelling FGFR2 with ab289968 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: SW620 (PMID: 24968263). Gated on viable cells.
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![Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)](https://www.abcam.co.jp/ps/products/289/ab289993/Images/ab289993-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-FGFR2 antibody [EPR24075-418] - BSA and Azide free (ab289993)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab289993 は論文での使用が確認できていません。
