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Anti-FGFR1 (phospho Y766) 抗体 (ab59180)

Reviews (1)Q&A (4)References (3)
Western blot - Anti-FGFR1 (phospho Y766) antibody (ab59180)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 (phospho Y766) antibody (ab59180)

Key features and details

  • Rabbit polyclonal to FGFR1 (phospho Y766)
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-FGFR1 (phospho Y766) antibody
    FGFR1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to FGFR1 (phospho Y766)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human FGFR1 aa 700-800 (phospho Y766).
    Database link: P11362

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab59180の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB (1)
1/500 - 1/1000.
特記事項
IHC-P
Use at an assay dependent concentration.
WB
1/500 - 1/1000.

ターゲット情報

  • 機能

    Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).

  • 組織特異性

    Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.

  • 関連疾患

    Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
    Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
    Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
    Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
    Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
    Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.

  • 細胞内局在

    Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Basic fibroblast growth factor receptor 1 antibody
    • bFGF-R-1 antibody
    • BFGFR antibody
    • CD331 antibody
    • CEK antibody
    • FGFBR antibody
    • FGFR 1 antibody
    • FGFR-1 antibody
    • FGFR1 antibody
    • FGFR1/PLAG1 fusion antibody
    • FGFR1_HUMAN antibody
    • fibroblast growth factor receptor 1 antibody
    • FLG antibody
    • FLT-2 antibody
    • FLT2 antibody
    • Fms-like gene antibody
    • Fms-like tyrosine kinase 2 antibody
    • fms-related tyrosine kinase 2 antibody
    • HBGFR antibody
    • heparin-binding growth factor receptor antibody
    • HH2 antibody
    • HRTFDS antibody
    • hydroxyaryl-protein kinase antibody
    • KAL2 antibody
    • N-SAM antibody
    • OGD antibody
    • Proto-oncogene c-Fgr antibody
    see all

画像

  • Western blot - Anti-FGFR1 (phospho Y766) antibody (ab59180)
    Western blot - Anti-FGFR1 (phospho Y766) antibody (ab59180)
    All lanes : Anti-FGFR1 (phospho Y766) antibody (ab59180)

    Lane 1 : EGF-treated HepG2 cell extract
    Lane 2 : EGF-treated HepG2 cell extract with blocking phosphopeptide
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 (phospho Y766) antibody (ab59180)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR1 (phospho Y766) antibody (ab59180)
    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab59180 with and without the addition of the synthetic phosphopeptide derived from human FGFR1 around the phosphorylation site of tyrosine 766.

参考文献 (3)

ab59180 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab59180 は 3 報の論文で使用されています。

  • Bunney TD  et al. Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System. Structure 26:446-458.e8 (2018). PubMed: 29478821
  • Pinotsis N & Waksman G Structure of the WipA protein reveals a novel tyrosine protein phosphatase effector from Legionella pneumophila. J Biol Chem 292:9240-9251 (2017). PubMed: 28389563
  • Zou L  et al. Fibronectin induces endothelial cell migration through ß1 integrin and Src-dependent phosphorylation of fibroblast growth factor receptor-1 at tyrosines 653/654 and 766. J Biol Chem 287:7190-202 (2012). WB ; Human, Mouse . PubMed: 22247553

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Western blot abreview for Anti-FGFR1 (phospho Y766) antibody

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U87 (Glioblastoma))
Loading amount
50 µg
Specification
U87 (Glioblastoma)
Treatment
siRNA FGFR1
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Nov 30 2011

Question

Hello, I would like to know if this antibody is specific against the phosphorylated form of FGFR1 or if it cross-reacts with the phosphorylated forms of the other FGFRs. Also, I am interested in antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs. Could you please let me know if you have any of those? Thanks.

Read More
Answer

Thank you for contacting us.

I have conducted a search through our catalogue for antibodies against pFGFR1 and pFGFR4 which do no cross-react with the other FGFRs.

Ab59180 is specific against FGFR1 when it is phosphorilated at Tyr766. Similarly, ab59194 is specific against FGFR1 when it is phosphorilated at Tyr654, while ab52165 is specific against FGFR1 when it is phosphorilated at Tyr154.

These three antibodies do not bind to non-phosphorilated proteins. If you would send me the accession numbers or the exact protein sequences of other FGFRs that you are concerned with cross reactivity, I can align them with our immunogen sequence for sequence homology and possible cross reactivity.

We do have other alternatives (ab111124 and ab134043) against pFGFR1. However, these antibodies may also detect FGFR2, FGFR3 and FGFR4 phosphorilated at the equivalent sites. Although the antibodies have not been tested to determine if it detects these other endogenous forms, based on sequence homology reactivity would be expected.

Unfortunately, all the https://www.abcam.com/Search?Keywords=FGFR4+&fReset=1&pt=1&source=TopSearch&btnSearch=Search are against non phosphorilated forms, so I am afraid they would not fit your requirements.

Please do not hesitate to let me know whether you require a proforma, or any further assistance.

Read More

Question

Many thanks for your reply.  Actually the order for this ab59180 antibody was made on *** and should be covered by the Abpromise policy. If possible, I would like to have a replacement for this antibody with this one: Anti-HIF2 alpha antibody (ab109731) It's exactly the same price (360 euros) Here is the reference of the previous order: *** For the delivery address: *** Many thanks Best regards

Read More
Answer

Thank you for your reply. I am sending a free of charge vial of ab109731 on the order *** which should arrive shortly. Please let me know if you have any questions or if there is anything else that we can do for you.  

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Question

AbID: 59180, Anti-FGFR1 (phospho Y766) antibody Rating: Below Average Image: Anti-FGFR1 (phospho Y766) antibody for Western blot (Human) Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 50 µg Specification: U87 (Glioblastoma) Treatment: siRNA FGFR1 Application: Application: Western blot Gel Running Conditions: Reduced Denaturing (10%) Blocking step: BSA as blocking agent for 20 mins at 20°C Blocking Concentration: 5% Other detail: Dilution: 1/800 Incubation time: 16 hours at 20°C Diluent: BSA 1% in TBS Tween 0.1% Secondary Antibody: Name: Non-Abcam Antibody was used: Bio-Rad Goat Anti-rabbit IgG (H+L) Conjugation: HRP Dilution: 1/10000 Detection: Detection method: ECL Exposure: 5 minutes and 0 seconds Bands: Specific: 120, 130 kDa Non-specific: 75, 100 kDa Additional Data: Additional Notes: Lane 1 : SiRNA control Lane 2 : SiRNA FGFR1 NB1: SiRNA FGFR1 works fine (80% inhibition by qPCR) NB2: Maybe this is due to a problem of specificity of this antibody with the different FGFR isoforms

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Answer

Thank you for submitting your recent review of ab59180. I am sorry to see that the results were so poor with this antibody. I do have a couple of suggestions that might help improve the results, however we do fully guarantee our products in tested species and applications for 6 months after purchase, so I would be happy to send a replacement antibody or issue a credit or refund. As you mentioned, some of the extra bands on the blot may be isoforms of the protein. I would recommend blocking for at least an hour and reducing the protein load to around 20-30 ug of protein to reduce non-specific bands. Also using a phosphatase inhibitor cocktail can ensure that the protein is sufficiently phosphorylated. We have tested the antibody in HepG2 cells treated with EGF, so a phosphorylating treatment may be necessary to see a strong band in the siRNA control. Please let me know if these suggestions are helpful, or if you would like to receive a replacement, credit, or refund please send me your original order number and I will be happy to arrange that  for you. I look forward to hearing from you.

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Question

Hi Coulf you tell me if this anti phosphoFGFR1 Ab and the other you have (ab59194, ab52165) are relatively specific for FGFR1 and do not cross-react with FGFR2/3/4 or other GF Receptors? many thanks Best regards

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Answer

Thank you for contacting us. Indeed, crossreactivity is always a very important information for an antibody. Sequence alignment and experimental data can help to predict/determine this. We receive these antibodies from a collaborating laboratory. I have asked the laboratory to know how the specificity has been determined and whether crossreactivity has experimentally been assessed. The laboratory had not assessed this crossreactivity experimentally. They have run a blast with the exact immunogen sequences (to which I have no access) and does on that basis exclude any possible crossreaction with other proteins than FGFR1. The regions from which the respective peptide immunogen of the antibodies are ab52165 (Tyr154), ab59180 (Tyr 766) and ab59194 (Tyr654). Please find here the sequence alignment for FGFR1 with FGFR2: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110907-091151-0127-47989414-oy For predicted crossreactivity with FGFR2: Comparison of the sequence with FGFR3 and FGFR1: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110907-103134-0638-15807362-pg Sequence alignment of FGFR1 with FGFR4: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20110907-103350-0499-7232840-pg The sequence around Tyr154 has deletions in the FGFR2 and FGFR3 sequence when compared to the sequence of FGFR1, resulting therefore in a very poor alignment of that region. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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