AB81384

Anti-FGF8 抗体

Anti-FGF8 antibody

JP Deleterious:医薬用外劇物

(1 Review)

(6 Publications)

Rabbit Polyclonal FGF8 antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human FGF8.

別名を表示する

AIGF, FGF8, Fibroblast growth factor 8, FGF-8, Androgen-induced growth factor, Heparin-binding growth factor 8, HBGF-8

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

ab81384 staining FGF8 in Human Ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 37°C; antigen retrieval was by microwave heat mediation in a citrate buffer. Samples were incubated with primary antibody (2 μg/mL) for 2 hours at 37°C. A Biotin-conjugated secondary antibody was used.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

ab81384 at 1μg/ml staining FGF8 in Rat ovary tissue sections by Immunohistochemistry(Formalin/ PFA-fixed paraffin-embedded sections). The tissue underwent heat mediated antigen retrieval. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.

Unknown

Western blot - Anti-FGF8 antibody (AB81384)

All lanes:

Western blot - Anti-FGF8 antibody (ab81384)

All lanes:

Rat Ovary Tissue Lysate

Predicted band size: 27 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human FGF8. The exact immunogen used to generate this antibody is proprietary information.

P55075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The FGF8 protein also known as fibroblast growth factor 8 plays a mechanical role in cell signaling and development. The FGF8 gene encodes two main isoforms: FGF8a and FGF8b each with different biological activities. FGF8 has a molecular mass of approximately 22-24 kDa. It expresses in developing tissues particularly in the embryo during early stages of vertebrate development. FGF8 interacts with fibroblast growth factor receptors (FGFRs) to initiate a cascade of signaling events leading to cellular responses.

Biological function summary

The FGF8 protein influences the regulation of cell division growth and differentiation particularly in embryonic development. FGF8 an integral part of several signaling complexes activates pathways that guide mesoderm and neural plate induction. Its expression is essential for limb and brain development providing necessary signaling cues required for proper patterning and morphogenetic processes.

Pathways

The FGF8 protein plays a central role in the MAPK/ERK signaling and PI3K/AKT pathways important mechanisms that govern cellular proliferation and survival. These pathways involve several proteins such as FGFR1 and FGFR2 which interact with FGF8 to mediate its effects. Through these pathways FGF8 coordinates with other members of the fibroblast growth factor family to regulate cellular functions in a concerted manner.

The FGF8 protein associates with various congenital malformations specifically conditions like Kallmann syndrome and certain craniofacial dysplasias. Mutations or dysregulation in FGF8 can disrupt normal signaling processes leading to abnormalities in development. The protein closely interacts with other growth factors and receptors such as FGFR1 which when altered might contribute to the pathogenesis of these disorders by affecting the signaling pathways they share with FGF8.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed : 16384934, PubMed : 16597617, PubMed : 8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed : 21576111).

See full target information FGF8

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of oral science 13:6 PubMed33649301

2021

FGF8 induces epithelial-mesenchymal transition and promotes metastasis in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yilong Hao,Yanxuan Xiao,Xiaoyu Liao,Shuya Tang,Xiaoyan Xie,Rui Liu,Qianming Chen

Journal of pediatric urology 16:41.e1-41.e10 PubMed31718875

2019

FGF8, FGF10 and FGF receptor 2 in foreskin of children with hypospadias: an analysis of immunohistochemical expression patterns and gene transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Bernhard Haid,Elisabeth Pechriggl,Felix Nägele,Jozsef Dudas,Gerald Webersinke,Melanie Rammer,Helga Fritsch,Josef Oswald

Oncology reports 38:2205-2210 PubMed28791365

2017

FGF8 promotes cell proliferation and resistance to EGFR inhibitors via upregulation of EGFR in human hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanmin Pei,Xueling Sun,Xiwei Guo,Huashan Yin,Le Wang,Fugu Tian,Hongxi Jing,Xiaobo Liang,Jun Xu,Pengcheng Shi

Cellular and molecular life sciences : CMLS 74:4561-4572 PubMed28726057

2017

Macrophage migration inhibitory factor (MIF) modulates trophic signaling through interaction with serine protease HTRA1.

Applications

ICC

Species

Mouse

Åsa Fex Svenningsen,Svenja Löring,Anna Lahn Sørensen,Ha Uyen Buu Huynh,Simone Hjæresen,Nellie Martin,Jesper Bonnet Moeller,Maria Louise Elkjær,Uffe Holmskov,Zsolt Illes,Malin Andersson,Solveig Beck Nielsen,Eirikur Benedikz

Microscopy research and technique 76:321-30 PubMed23362007

2013

Fibroblast growth factor, estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cia Hetzl,Fabio Montico,Raisa Misitieri Lorencini,Larissa Kido,Eduardo Cândido,Athanase Billis,Ubirajara Ferreira,Valeria Ha Cagnon

Integrative biology : quantitative biosciences fro 3:1179-87 PubMed22028041

2011

Spatially organized in vitro models instruct asymmetric stem cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chin Toh,Katarina Blagovic,Hanry Yu,Joel Voldman

View all publications

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Abcam product promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-FGF8 抗体

Anti-FGF8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF8 antibody (AB81384)

Western blot - Anti-FGF8 antibody (AB81384)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise