Anti-FGF5 抗体 (ab88118)
Key features and details
- Rabbit polyclonal to FGF5
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FGF5 antibody
FGF5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FGF5 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
-
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Cow, Dog
-
免疫原
Synthetic peptide corresponding to Human FGF5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab106210) -
ポジティブ･コントロール
- Recombinant human FGF5 protein (ab50229) can be used as a positive control in WB. This anitbody gave a positive signal in the following tissues lysates: E14 Mouse Embryo Brain; E16 Ms Embryo Brain; E18 Ms Embryo Brain ICC/IF: NIH3T3 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88118の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase. -
組織特異性
Expressed in neonatal brain. -
配列類似性
Belongs to the heparin-binding growth factors family. -
発生段階
Can transform NIH 3T3 cells. -
細胞内局在
Secreted. - Information by UniProt
-
参照データベース
- Entrez Gene: 536771 Cow
- Entrez Gene: 2250 Human
- Entrez Gene: 14176 Mouse
- Omim: 165190 Human
- SwissProt: A0MTF4 Cow
- SwissProt: P12034 Human
- SwissProt: P15656 Mouse
- Unigene: 37055 Human
see all -
別名
- FGF 5 antibody
- FGF-5 antibody
- FGF5 antibody
see all
画像
-
Immunocytochemistry/ Immunofluorescence - Anti-FGF5 antibody (ab88118)
ab88118 staining FGF5 in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab88118 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
Western blot - Anti-FGF5 antibody (ab88118)All lanes : Anti-FGF5 antibody (ab88118) at 1 µg/ml
Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
Lane 2 : E16 Ms Embryo Brain Tissue Lysate
Lane 3 : E18 Ms Embryo Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab88118 は 6 報の論文で使用されています。
- Ban Q et al. Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency. iScience 26:106446 (2023). PubMed: 37091250
- Dai Y et al. Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C. Cell Death Discov 8:344 (2022). PubMed: 35915078
- Vila-Cejudo M et al. Wnt pathway modulation generates blastomere-derived mouse embryonic stem cells with different pluripotency features. J Assist Reprod Genet 37:2967-2979 (2020). PubMed: 33047186
- Chen B et al. FGF5 Regulates Schwann Cell Migration and Adhesion. Front Cell Neurosci 14:237 (2020). PubMed: 32848626
- Dai Y et al. Methyl-CpG-binding domain 3 (Mbd3) is an important regulator for apoptosis in mouse embryonic stem cells. Am J Transl Res 12:8147-8161 (2020). PubMed: 33437388
- Ding BS et al. Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. J Cell Sci 128:2983-8 (2015). IHC ; Human . PubMed: 26116571