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Anti-FGF 23 抗体 (ab56326)

Submit a review Submit a question References (11)
Western blot - Anti-FGF 23 antibody (ab56326)
  • Immunocytochemistry/ Immunofluorescence - Anti-FGF 23 antibody (ab56326)

Key features and details

  • Goat polyclonal to FGF 23
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-FGF 23 antibody
    FGF 23 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to FGF 23

  • 由来種

    Goat

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide:

    C-RHTRSAEDDSERD

    , corresponding to internal sequence amino acids 176-188 of Human FGF 23
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Human Brain (Hippocampus) lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56326の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).

A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide.
ICC/IF
Use a concentration of 1 µg/ml.
特記事項
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).

A 1 hour primary incubation is recommended for this product. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunising peptide.
ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.

  • 組織特異性

    Expressed in osteogenic cells particularly during phases of active bone remodeling. In adult trabecular bone, expressed in osteocytes and flattened bone-lining cells (inactive osteoblasts).

  • 関連疾患

    Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) [MIM:193100]. ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses.
    Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues.

  • 配列類似性

    Belongs to the heparin-binding growth factors family.

  • 翻訳後修飾

    Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases.
    O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23.

  • 細胞内局在

    Secreted. Secretion is dependent on O-glycosylation.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADHR antibody
      • FGF-23 antibody
      • Fgf23 antibody
      • FGF23_HUMAN antibody
      • FGFN antibody
      • Fibroblast growth factor 23 antibody
      • Fibroblast growth factor 23 C-terminal peptide antibody
      • Fibroblast growth factor 23 precursor antibody
      • HPDR2 antibody
      • HYPF antibody
      • Phosphatonin antibody
      • PHPTC antibody
      • Tumor derived hypophosphatemia inducing factor antibody
      • Tumor-derived hypophosphatemia-inducing factor antibody
      see all

    画像

    • Western blot - Anti-FGF 23 antibody (ab56326)
      Western blot - Anti-FGF 23 antibody (ab56326)
      Anti-FGF 23 antibody (ab56326) at 0.5 µg/ml + Human Brain (Hippocampus) lysate (35µg protein in RIPA buffer)

      Predicted band size: 28 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?
      Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.



      Primary incubation was 1 hour. Detected by chemiluminescence.
    • Immunocytochemistry/ Immunofluorescence - Anti-FGF 23 antibody (ab56326)
      Immunocytochemistry/ Immunofluorescence - Anti-FGF 23 antibody (ab56326)
      ICC/IF image of ab56326 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56326, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    参考文献 (11)

    ab56326 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56326 は 11 報の論文で使用されています。

    • Fang L  et al. FGF23 promotes proliferation, migration and invasion by regulating miR-340-5p in osteosarcoma. J Orthop Surg Res 18:12 (2023). PubMed: 36604721
    • Balogh DB  et al. Cardioprotective and Antifibrotic Effects of Low-Dose Renin-Angiotensin-Aldosterone System Inhibitors in Type 1 Diabetic Rat Model. Int J Mol Sci 24:N/A (2023). PubMed: 38069366
    • Leow MKS  et al. Paraneoplastic Secretion of Multiple Phosphatonins From a Deep Fibrous Histiocytoma Causing Oncogenic Osteomalacia. J Clin Endocrinol Metab 106:e2299-e2308 (2021). PubMed: 33462615
    • Ursem SR  et al. Localization of fibroblast growth factor 23 protein in the rat hypothalamus. Eur J Neurosci 54:5261-5271 (2021). PubMed: 34184338
    • Li D  et al. Mg-Zn-Mn alloy extract induces the angiogenesis of human umbilical vein endothelial cells via FGF/FGFR signaling pathway. Biochem Biophys Res Commun 514:618-624 (2019). PubMed: 31076107
    View all Publications for this product

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