Anti-Ferritin Light Chain 抗体 (ab186871)
Key features and details
- Rabbit polyclonal to Ferritin Light Chain
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Ferritin Light Chain antibody
Ferritin Light Chain 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ferritin Light Chain -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Cat, Dog, Orangutan
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免疫原
Recombinant full length protein corresponding to Human Ferritin Light Chain aa 1 to the C-terminus.
Database link: P02792 -
ポジティブ･コントロール
- H460, BT474, 239T, MCF7 and HepG2 cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Predicted molecular weight: 20 kDa.
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| IHC-P | (1) |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
WB
1/500 - 1/2000. Predicted molecular weight: 20 kDa. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. -
関連疾患
Defects in FTL are the cause of hereditary hyperferritinemia-cataract syndrome (HHCS) [MIM:600886]. It is an autosomal dominant disease characterized by early-onset bilateral cataract. Affected patients have elevated level of circulating ferritin. HHCS is caused by mutations in the iron responsive element (IRE) of the FTL gene.
Defects in FTL are the cause of neurodegeneration with brain iron accumulation type 3 (NBIA3) [MIM:606159]; also known as adult-onset basal ganglia disease. It is a movement disorder with heterogeneous presentations starting in the fourth to sixth decade. It is characterized by a variety of neurological signs including parkinsonism, ataxia, corticospinal signs, mild nonprogressive cognitive deficit and episodic psychosis. It is linked with decreased serum ferritin levels. -
配列類似性
Belongs to the ferritin family.
Contains 1 ferritin-like diiron domain. - Information by UniProt
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参照データベース
- Entrez Gene: 477042 Dog
- Entrez Gene: 2512 Human
- Entrez Gene: 100173858 Orangutan
- Omim: 134790 Human
- SwissProt: Q53VB8 Dog
- SwissProt: P02791 Horse
- SwissProt: P02792 Human
- SwissProt: Q5R538 Orangutan
see all -
別名
- Ferritin L chain antibody
- Ferritin L subunit antibody
- Ferritin light chain antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ferritin Light Chain antibody (ab186871)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling Ferritin Light Chain with ab186871 at 1/100. Magnification: 200x.
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Western blot - Anti-Ferritin Light Chain antibody (ab186871)All lanes : Anti-Ferritin Light Chain antibody (ab186871) at 1/500 dilution
Lane 1 : H460 cell extract
Lane 2 : BT474 cell extract
Lane 3 : 293T cell extract
Lane 4 : MCF7 cell extract
Lane 5 : HepG2 cell extract
Predicted band size: 20 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab186871 は 1 報の論文で使用されています。
- Kobak KA et al. Primary Human Cardiomyocytes and Cardiofibroblasts Treated with Sera from Myocarditis Patients Exhibit an Increased Iron Demand and Complex Changes in the Gene Expression. Cells 10:N/A (2021). PubMed: 33917391