Anti-FER (phospho Y402) 抗体 (ab79573)
Key features and details
- Rabbit polyclonal to FER (phospho Y402)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-FER (phospho Y402) antibody
FER 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FER (phospho Y402) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
-
免疫原
Synthetic peptide corresponding to Human FER (phospho Y402).
-
ポジティブ･コントロール
- Human brain tissue; Jurkat cell extracts starved for 24 hours.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab79573 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79573の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 93 kDa.
|
|
| IHC-P |
1/50 - 1/100.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 93 kDa. |
|
IHC-P
1/50 - 1/100. |
ターゲット情報
-
機能
Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control. -
組織特異性
Expressed in a variety of lymphoid cell lines. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.
Contains 1 FCH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain. -
細胞内局在
Cytoplasm. Nucleus. Associated with the chromatin. - Information by UniProt
-
参照データベース
- Entrez Gene: 2241 Human
- Entrez Gene: 14158 Mouse
- Omim: 176942 Human
- SwissProt: P16591 Human
- SwissProt: P70451 Mouse
- Unigene: 221472 Human
- Unigene: 23039 Mouse
-
別名
- c FER antibody
- Feline encephalitis virus related kinase FER antibody
- Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94) antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FER (phospho Y402) antibody (ab79573)ab79573, at a 1/50 dilution, staining FER in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.
-
Western blot - Anti-FER (phospho Y402) antibody (ab79573)All lanes : Anti-FER (phospho Y402) antibody (ab79573) at 1/500 dilution
Lane 1 : Jurkat cell extracts, starved (24 hours)
Lane 2 : Jurkat cell extracts, starved (24 hours) with immunizing phospho peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab79573 は 3 報の論文で使用されています。
- Zhang Y et al. Development of the nonreceptor tyrosine kinase FER-targeting PROTACs as a potential strategy for antagonizing ovarian cancer cell motility and invasiveness. J Biol Chem 299:104825 (2023). PubMed: 37196766
- Amtul Z et al. Comorbid rat model of ischemia and β-amyloid toxicity: striatal and cortical degeneration. Brain Pathol 25:24-32 (2015). PubMed: 24725245
- Chang SF et al. Different modes of endothelial-smooth muscle cell interaction elicit differential ß-catenin phosphorylations and endothelial functions. Proc Natl Acad Sci U S A 111:1855-60 (2014). PubMed: 24449884