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AB109132

Anti-FEN1 抗体 [EPR4460(2)]

Anti-FEN1 antibody [EPR4460(2)]

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(11 Publications)

Rabbit Recombinant Monoclonal FEN1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 11 publications.

別名を表示する

RAD2, FEN1, Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (AB109132)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (AB109132)

Immunohistochemical analysis of paraffin-embedded Human urothelial carcinoma tissue using ab109132 at a dilution of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-FEN1 antibody [EPR4460(2)] (AB109132)
  • WB

Unknown

Western blot - Anti-FEN1 antibody [EPR4460(2)] (AB109132)

All lanes:

Western blot - Anti-FEN1 antibody [EPR4460(2)] (ab109132) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Y79 cell lysate at 10 µg

Lane 4:

Daudi cell lysate at 10 µg

Predicted band size: 43 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (AB109132)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (AB109132)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109132 at a dilution of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (AB109132)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody [EPR4460(2)] (AB109132)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109132 at a dilution of 1/500.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-FEN1 antibody [EPR4460(2)] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4460(2)

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Flap Endonuclease 1 known as FEN1 or FEN-1 is an important protein involved in DNA replication and repair. This protein shows a molecular weight of approximately 42 kDa. FEN1 functions as an endonuclease where it removes 5’ overhanging flaps in DNA products that occur during DNA replication and repair processes. It expresses in a wide range of human tissues with significant presence in the nucleus where DNA synthesis mainly occurs.
Biological function summary

FEN1 plays an important role in the Okazaki fragment maturation during lagging strand synthesis and long-patch base excision repair. FEN1 is not a solitary actor; it forms a part of larger complexes essential for maintaining genomic stability. It works closely with proliferating cell nuclear antigen (PCNA) to enhance its function and to interact with other proteins essential for the stability of the DNA.

Pathways

DNA replication and repair processes heavily rely on the activity of FEN1. It’s a component of the DNA replication fork protection and repair pathway and interacts with other proteins such as DNA polymerase and DNA ligase I to complete the repair process. Efficient DNA replication ensures accurate genomic duplication preventing mutations that could lead to severe cellular malfunction.

FEN1 is linked to the development of cancer and neurodegenerative diseases like Huntington's disease. FEN1 mutations or malfunction can result in genomic instability and an increased risk of tumorigenesis. In the context of neurodegenerative diseases deficiencies in FEN1 activity may disrupt the repair of oxidative DNA damage which is an important feature of disorders like Huntington's. These insights highlight the need for further research into FEN1's role and interactions to develop potential therapeutic strategies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
See full target information FEN1
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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 30:1434-1445 PubMed37580626

2023

Fanconi anemia DNA crosslink repair factors protect against LINE-1 retrotransposition during mouse development.

Applications

Unspecified application

Species

Unspecified reactive species

Nazareno Bona,Gerry P Crossan

Haematologica 108:1092-1104 PubMed36519324

2023

Overexpression of the key metabolic protein CPT1A defines mantle cell lymphoma patients with poor response to standard high-dose chemotherapy independent of MIPI and complement established highrisk factors.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Sandström Gerdtsson,Joana de Matos Rodrigues,Christian Winther Eskelund,Simon Husby,Kirsten Grønbæk,Riikka Räty,Arne Kolstad,Christian Geisler,Anna Porwit,Mats Jerkeman,Sara Ek

Molecular cell 81:3128-3144.e7 PubMed34216544

2021

Replication gaps are a key determinant of PARP inhibitor synthetic lethality with BRCA deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Cong,Min Peng,Arne Nedergaard Kousholt,Wei Ting C Lee,Silviana Lee,Sumeet Nayak,John Krais,Pamela S VanderVere-Carozza,Katherine S Pawelczak,Jennifer Calvo,Nicholas J Panzarino,John J Turchi,Neil Johnson,Jos Jonkers,Eli Rothenberg,Sharon B Cantor

Molecular & cellular proteomics : MCP 20:100015 PubMed33508502

2021

Proteomic Analyses Identify Differentially Expressed Proteins and Pathways Between Low-Risk and High-Risk Subtypes of Early-Stage Lung Adenocarcinoma and Their Prognostic Impacts.

Applications

Unspecified application

Species

Unspecified reactive species

Juntuo Zhou,Bing Liu,Zhongwu Li,Yang Li,Xi Chen,Yuanyuan Ma,Shi Yan,Xin Yang,Lijun Zhong,Nan Wu

Frontiers in oncology 10:578816 PubMed33224879

2020

IGF2BP2 Promotes Liver Cancer Growth Through an m6A-FEN1-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Pu,Jianchu Wang,Zebang Qin,Anmin Wang,Ya Zhang,Xianjian Wu,Yi Wu,Wenchuan Li,Zuoming Xu,Yuan Lu,Qianli Tang,Huamei Wei

Molecular medicine reports 22:4919-4930 PubMed33173950

2020

miR‑140‑5p inhibits cervical cancer cell phenotypes via downregulating FEN1 to halt the cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Yanrong Guo,Shihua Luo

BMC complementary medicine and therapies 20:196 PubMed32586310

2020

Jianpi-yangwei decoction inhibits DNA damage repair in the drug resistance of gastric cancer by reducing FEN1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Huang,Huijuan Tang,Fang Wen,Xiaona Lu,Qingpei Li,Peng Shu

Aging 10:2062-2078 PubMed30144787

2018

LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Siping Ma,Dongxiang Yang,Yanlong Liu,Yongpeng Wang,Tao Lin,Yanxi Li,Shihua Yang,Wanchuan Zhang,Rui Zhang

Pathology, research and practice 214:840-847 PubMed29735403

2018

Proteins of the retinoblastoma pathway, FEN1 and MGMT are novel potential prognostic biomarkers in pancreatic adenocarcinoma.

Applications

IHC-P

Species

Human

Joel Isohookana,Kirsi-Maria Haapasaari,Ylermi Soini,Joni Leppänen,Peeter Karihtala

PloS one 12:e0179278 PubMed28628639

2017

Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes.

Applications

WB

Species

Human

Thomas A Ward,Peter J McHugh,Stephen T Durant
View all publications
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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