Anti-FEN1 抗体 (ab153825)
Key features and details
- Rabbit polyclonal to FEN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FEN1 antibody
FEN1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FEN1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to a region within amino acids 169 and 380 of Human FEN1 (UniProt: P39748).
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ポジティブ･コントロール
- Recombinant Human FEN1 protein (ab95382) can be used as a positive control in WB. HepG2, NIH 3T3, JC, BCL1, Raw264.7 and PC12 whole cell lysates; A549 cells; A549 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153825の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. -
配列類似性
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. -
翻訳後修飾
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.
Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.
Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA. -
細胞内局在
Nucleus > nucleolus. Nucleus > nucleoplasm. Mitochondrion. Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 2237 Human
- Entrez Gene: 14156 Mouse
- Entrez Gene: 84490 Rat
- Omim: 600393 Human
- SwissProt: P39748 Human
- SwissProt: P39749 Mouse
- SwissProt: Q5XIP6 Rat
- SwissProt: Q9JHW7 Rat
see all -
別名
- DNase IV antibody
- FEN-1 antibody
- FEN1 antibody
see all
画像
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Western blot - Anti-FEN1 antibody (ab153825)Anti-FEN1 antibody (ab153825) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Western blot - Anti-FEN1 antibody (ab153825)All lanes : Anti-FEN1 antibody (ab153825) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : Raw264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 43 kDa
10% SDS-PAGE -
Western blot - Anti-FEN1 antibody (ab153825)Anti-FEN1 antibody (ab153825) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FEN1 antibody (ab153825)Immunofluorescence analysis of paraformaldehyde fixed A549 cells labeling FEN1 with ab153825 at 1/200. The lower panel is merged with DNA probe. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FEN1 antibody (ab153825)Immunohistochemical analysis of paraffin embedded A549 xenograft tissue labeling FEN1 with ab153825 at 1/100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab153825 は 2 報の論文で使用されています。
- Wu H et al. miR-4324 inhibits ovarian cancer progression by targeting FEN1. J Ovarian Res 15:32 (2022). PubMed: 35246224
- Asada K et al. Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing. Nucleic Acids Res 49:4864-4876 (2021). PubMed: 33928345