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AB321968

Anti-FCRL5 抗体 [EPR26948-187]

Anti-FCRL5 antibody [EPR26948-187]

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Anti-FCRL5 antibody [EPR26948-187] is a Rabbit Monoclonal antibody that is used in FCRL5 Flow Cytometry. Suitable for Human samples.

FCRL5 (Fc receptor-like protein 5) is a protein encoded by the FCRL5 gene, also known as CD307. It plays a role in the immune system, particularly in B cell activation and regulation. FCRL5 is associated with diseases such as hairy cell leukemia and lymphoma.

別名を表示する

CD307e, FCRH5, IRTA2, UNQ503/PRO820, FCRL5, Fc receptor-like protein 5, FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, Immune receptor translocation-associated protein 2, FcRH5

4 Images
Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast) (Right) / MOLT-4 (human lymphoblastic leukemia T lymphoblast) (Left) cells labelling FCRL5 with ab321968 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647 ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : MOLT-4 (PMID : 11290337).
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2 FCRL3 FCRL4 and FCRL6.

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling FCRL5 with ab321968 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488 ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD3 conjugated to Brilliant Violet 421. Gated on viable Lymphocytes.
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2 FCRL3 FCRL4 and FCRL6.

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)

Flow cytometric analysis of NAMALWA (human Burkitt's lymphoma B lymphocyte) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling FCRL5 with ab321968 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647 ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : Jurkat (PMID : 24504816).
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2 FCRL3 FCRL4 and FCRL6.

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] (AB321968)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling FCRL5 with ab321968 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488 ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD19 conjugated to Alexa Fluor 647. Gated on viable Lymphocytes.
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2 FCRL3 FCRL4 and FCRL6.

関連する標識済み抗体及び組成の異なる製品 (1)

  • 578 PE

    PE Anti-FCRL5 antibody [EPR26948-187]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR26948-187

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The FCRL5 also known as CD307 or Fc receptor-like 5 is a protein predominantly expressed on the surface of B cells and plasma cells. This protein weighs approximately 98 kDa. FCRL5 features structural similarities to classical Fc receptors suggesting a role in immune responses. Its expression is tightly regulated throughout B cell development and has been detected in lymphoid tissues with higher levels reported in bone marrow and secondary lymphoid organs.
Biological function summary

FCRL5 modulates the immune system by influencing B cell activation and differentiation. It participates in the formation of immune complexes playing a dual role in both promoting and inhibiting immune responses depending on the surrounding conditions. This regulatory function suggests that FCRL5 can adjust immune system activities to maintain homeostasis. It acts independently and might form complexes with other immune regulatory proteins to fine-tune B cell responses.

Pathways

FCRL5 plays a role in the adaptive immune response and is associated with the B cell receptor (BCR) signaling pathway. It interacts with proteins such as CD19 and CD79 influencing downstream signaling cascades critical for B cell maturation and effector functions. The interaction of FCRL5 with these pathways highlights its importance in modulating signal transduction that leads to cytokine production and antibody generation.

FCRL5 has been implicated in the pathogenesis of autoimmune diseases and certain hematological cancers such as chronic lymphocytic leukemia (CLL). Abnormal expression or mutations of FCRL5 can dysregulate normal B cell function contributing to disease development. In CLL FCRL5 potentially interacts with proteins like leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) influencing cancer cell survival and proliferation making it a target for therapeutic intervention and a potential biomarker for disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays an important role in B-cell response to antigen that acts both as a negative or positive coreceptor. Inhibits B-cell receptor (BCR) signaling in the absence of CR2 stimulation but engagement with CR2 and the BCR lead to a superior calcium response compared to CR2 and BCR costimulation (PubMed : 30107486). May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells. May play a role in fertilization (By similarity).
See full target information FCRL5
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