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リコンビナントRabMAb

Anti-Fbxw7 抗体 [SP237] - BSA and Azide free (ab246353)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
  • Flow Cytometry (Intracellular) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
  • Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP237] to Fbxw7 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Fbxw7 antibody [SP237] - BSA and Azide free
    Fbxw7 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP237] to Fbxw7 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human endometrial carcinoma, and Human endometrial adenocarcinoma tissue; Flow Cyt (intra): HL-60 cells.

  • 特記事項

    ab246353 is the carrier-free version of ab227677.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab246353の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Antigen retrieval: Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

 
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Antigen retrieval: Boil tissue section in EDTA buffer pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

 

ターゲット情報

  • 機能

    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. Involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.

  • 組織特異性

    Isoform 1 is widely expressed. Isoform 4 is expressed in brain.

  • 配列類似性

    Contains 1 F-box domain.
    Contains 7 WD repeats.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Cytoplasm; Nucleus; Nucleus > nucleolus and Nucleus > nucleoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AGO antibody
      • Archipelago antibody
      • Archipelago homolog antibody
      • Archipelago, Drosophila, homolog of antibody
      • CDC4 antibody
      • CDC4, S.cerevisiae, homolog of antibody
      • DKFZp686F23254 antibody
      • F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody
      • F box and WD 40 domain protein 7 antibody
      • F box and WD repeat domain containing 7 antibody
      • F box protein FBW7 antibody
      • F box protein FBX30 antibody
      • F box protein SEL 10 antibody
      • F box protein SEL10 antibody
      • F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody
      • F-box and WD-40 domain-containing protein 7 antibody
      • F-box protein FBX30 antibody
      • F-box/WD repeat-containing protein 7 antibody
      • FBW6 antibody
      • FBW7 antibody
      • FBX30 antibody
      • FBXO30 antibody
      • FBXW6 antibody
      • FBXW7 antibody
      • FBXW7_HUMAN antibody
      • FLJ16457 antibody
      • hAgo antibody
      • hCdc4 antibody
      • Homolog of C elegans sel 10 antibody
      • Homolog of C.elegans sel10 antibody
      • SEL 10 antibody
      • SEL-10 antibody
      • SEL10 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human endometrial adenocarcinoma tissue sections labeling Fbxw7 with ab227677 at 1/100 dilution (1.56 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227677)
    • Flow Cytometry (Intracellular) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
      Flow Cytometry (Intracellular) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)

      Intracellular flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Fbxw7 with ab227677 at 1/400 (green) compared with a rabbit isotype control (blue). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227677). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)

      Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for Fbxw7 with ab227677 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227677).

    • Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)
      Anti-Fbxw7 antibody [SP237] - BSA and Azide free (ab246353)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab246353 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab246353 は論文での使用が確認できていません。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com