Anti-FBXO4/FBX4 抗体 (ab153803)
Key features and details
- Rabbit polyclonal to FBXO4/FBX4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FBXO4/FBX4 antibody -
製品の詳細
Rabbit polyclonal to FBXO4/FBX4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to Human FBXO4/FBX4 aa 24-318.
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ポジティブ･コントロール
- HL60 whole cell lysates; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153803の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 F-box domain. -
翻訳後修飾
Phosphorylation at Ser-12 varies during the cell cycle. It is low in resting cells and high in the S phase and the G2/M phase of the cell cycle. Phosphorylation is decreased during late G1 phase (By similarity). Phosphorylation at Ser-12 promotes homodimerization and is necessary for optimal ubiquitin ligase activity towards CCND1. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 534852 Cow
- Entrez Gene: 26272 Human
- Entrez Gene: 106052 Mouse
- Entrez Gene: 310363 Rat
- Omim: 609090 Human
- SwissProt: Q3T0J1 Cow
- SwissProt: Q9UKT5 Human
- SwissProt: Q8CHQ0 Mouse
see all -
別名
- DKFZp547N213 antibody
- F box only protein 4 antibody
- F box protein 4 antibody
see all
画像
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Western blot - Anti-FBXO4/FBX4 antibody (ab153803)Anti-FBXO4/FBX4 antibody (ab153803) at 1/5000 dilution + HL60 whole cell
lysate
at 30 µg
Predicted band size: 44 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-FBXO4/FBX4 antibody (ab153803)Immunofluorescent analysis of methanol-fixed HeLa cells labeling FBXO4/FBX4 with ab153803 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab153803 は 2 報の論文で使用されています。
- Wang B et al. circNRIP1 facilitates keloid progression via FXR1-mediated upregulation of miR-503-3p and miR-503-5p. Int J Mol Med 47:N/A (2021). PubMed: 33649815
- Gong Y et al. Pan-cancer genetic analysis identifies PARK2 as a master regulator of G1/S cyclins. Nat Genet 46:588-94 (2014). PubMed: 24793136