Anti-FBXO31 抗体 (ab86137)
Key features and details
- Rabbit polyclonal to FBXO31
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FBXO31 antibody
FBXO31 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FBXO31 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human FBXO31 aa 450-550.
Database link: Q5XUX0 -
ポジティブ･コントロール
- Whole cell lysate from 293T and mouse NIH3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86137の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
ターゲット情報
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機能
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. Specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. May act as a tumor suppressor. -
組織特異性
Highly expressed in brain. Expressed at moderate levels in most tissues, except bone marrow. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the FBXO31 family.
Contains 1 F-box domain. -
発生段階
Expression is cell-cycle regulated, and peaks at late G2 to early G1 phase (at protein level). -
翻訳後修飾
Phosphorylation at Ser-278 by ATM following gamma-irradiation results in its stabilization. - Information by UniProt
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参照データベース
- Entrez Gene: 79791 Human
- Entrez Gene: 76454 Mouse
- Omim: 609102 Human
- SwissProt: Q5XUX0 Human
- SwissProt: Q3TQF0 Mouse
- Unigene: 567582 Human
- Unigene: 467749 Mouse
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別名
- DKFZp434B027 antibody
- DKFZp434J1815 antibody
- F box only protein 31 antibody
see all
画像
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Western blot - Anti-FBXO31 antibody (ab86137)All lanes : Anti-FBXO31 antibody (ab86137) at 0.4 µg/ml
Lane 1 : Whole cell lysate from 293T cells
Lane 2 : Whole cell lysate from mouse NIH3T3 cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab86137 は 5 報の論文で使用されています。
- Liu J et al. Pseudophosphatase STYX is induced by Helicobacter pylori and promotes gastric cancer progression by inhibiting FBXO31 function. Cell Death Dis 13:268 (2022). PubMed: 35338113
- Zou S et al. FBXO31 Suppresses Gastric Cancer EMT by Targeting Snail1 for Proteasomal Degradation. Mol Cancer Res 16:286-295 (2018). PubMed: 29117943
- Liu J et al. Overexpression of F-box only protein 31 predicts poor prognosis and deregulates p38a- and JNK-mediated apoptosis in esophageal squamous cell carcinoma. Int J Cancer 142:145-155 (2018). PubMed: 28905993
- Abdullah A et al. Murine Myocardial Transcriptome Analysis Reveals a Critical Role of COPS8 in the Gene Expression of Cullin-RING Ligase Substrate Receptors and Redox and Vesicle Trafficking Pathways. Front Physiol 8:594 (2017). WB ; Mouse . PubMed: 28861005
- Liu J et al. F-box only protein 31 (FBXO31) negatively regulates p38 mitogen-activated protein kinase (MAPK) signaling by mediating lysine 48-linked ubiquitination and degradation of mitogen-activated protein kinase kinase 6 (MKK6). J Biol Chem 289:21508-18 (2014). ICC/IF ; Human . PubMed: 24936062