Anti-FBXL20 抗体 (ab67232)
Key features and details
- Rabbit polyclonal to FBXL20
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-FBXL20 antibody -
製品の詳細
Rabbit polyclonal to FBXL20 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to Human FBXL20 (C terminal).
Database link: Q96IG2 -
ポジティブ･コントロール
- A20 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67232の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 44, 48 kDa (predicted molecular weight: 44 , 48 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 44, 48 kDa (predicted molecular weight: 44 , 48 kDa). |
ターゲット情報
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関連性
FBXL20 is a member of the F-box protein family, which are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. FBXL20 is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. FBXL20 has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. FBXL20 is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 72194 Mouse
- SwissProt: Q9CZV8 Mouse
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別名
- F box and leucine rich repeat protein 20 antibody
- F box/LRR repeat protein 2 like antibody
- F box/LRR repeat protein 20 antibody
see all
画像
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Western blot - Anti-FBXL20 antibody (ab67232)Lane 1 : Anti-FBXL20 antibody (ab67232) at 0.5 µg/ml
Lane 2 : Anti-FBXL20 antibody (ab67232) at 1 µg/ml
Lane 1 : A20 (Mouse reticulum sarcoma cell line) whole cell lysate
Lane 2 : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 44 , 48 kDa
Observed band size: 44,48 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab67232 は論文での使用が確認できていません。