Anti-FBXL2 抗体 (ab17018)
Key features and details
- Goat polyclonal to FBXL2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-FBXL2 antibody
FBXL2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to FBXL2 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide: VFSNNDEGLINKK, corresponding to N terminal amino acids 2-14 of Human FBXL2.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17018の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Calcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication. -
組織特異性
Expressed in brain, heart, kidney, liver, lung, pancreas and placenta. -
配列類似性
Contains 1 F-box domain.
Contains 13 LRR (leucine-rich) repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 616212 Cow
- Entrez Gene: 25827 Human
- Entrez Gene: 363156 Rat
- Omim: 605652 Human
- SwissProt: A6H779 Cow
- SwissProt: Q9UKC9 Human
- Unigene: 475872 Human
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別名
- DKFZP564P0622 antibody
- F box and leucine rich repeat protein 2 antibody
- F box protein containing leucine rich repeats antibody
see all
画像
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Western blot - Anti-FBXL2 antibody (ab17018)All lanes : Anti-FBXL2 antibody (ab17018) at 0.3 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 49 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Western blot - Anti-FBXL2 antibody (ab17018)ab17018 at 0.5µ g/ml detecting FBXL2 from Human kidney lysate (35µ g total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence. ab17018 at 0.5µg/ml detecting FBXL2 from Human kidney lysate (35µg total protein per lane). Primary antibody was incubated for 1 hour and the blot was detected using chemiluminescence.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab17018 は 2 報の論文で使用されています。
- Liao C et al. Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer. Cancer Discov 10:1706-1721 (2020). PubMed: 32690540
- Yu Q et al. miRNA-346 promotes proliferation, migration and invasion in liver cancer. Oncol Lett 14:3255-3260 (2017). PubMed: 28927074